Introduction / objectives Methicillin-resistant Staphylococcus aureus (MRSA) is an increasing problem in the Americas and the Caribbean including Cuba. Recently, MRSA isolates are emerging as significant pathogens in the community. In the USA, the most prevalent community associated (CA-)MRSA clone is USA300 (ST8, spa type 008). Little is known about the molecular epidemiology of MRSA in some of the Caribbean countries. In this study we aim to investigate the molecular epidemiology of MRSA isolates from 4 major Cuban hospitals.