Methicillin-resistant Staphylococcus aureus in Spain: molecular epidemiology and utility of different typing methods.

@article{Vindel2009MethicillinresistantSA,
  title={Methicillin-resistant Staphylococcus aureus in Spain: molecular epidemiology and utility of different typing methods.},
  author={Ana Vindel and O. Cuevas and Emilia Cercenado and Carmen Marcos and Ver{\'o}nica Bautista and Carol Castellares and Pilar Trincado and Teresa Boquete and Mar{\'i}a Teresa P{\'e}rez-V{\'a}zquez and Mercedes Mar{\'i}n and Emilio Bouza},
  journal={Journal of clinical microbiology},
  year={2009},
  volume={47 6},
  pages={1620-7}
}
In a point-prevalence study performed in 145 Spanish hospitals in 2006, we collected 463 isolates of Staphylococcus aureus in a single day. Of these, 135 (29.2%) were methicillin (meticillin)-resistant S. aureus (MRSA) isolates. Susceptibility testing was performed by a microdilution method, and mecA was detected by PCR. The isolates were analyzed by pulsed-field gel electrophoresis (PFGE) after SmaI digestion, staphylococcal chromosomal cassette mec (SCCmec) typing, agr typing, spa typing with… CONTINUE READING