Methanotrophic bacteria in warm geothermal spring sediments identified using stable-isotope probing.

@article{Sharp2014MethanotrophicBI,
  title={Methanotrophic bacteria in warm geothermal spring sediments identified using stable-isotope probing.},
  author={Christine E. Sharp and Azucena Martinez-Lorenzo and Allyson L Brady and Stephen E. Grasby and Peter F. Dunfield},
  journal={FEMS microbiology ecology},
  year={2014},
  volume={90 1},
  pages={
          92-102
        }
}
We investigated methanotrophic bacteria in sediments of several warm geothermal springs ranging in temperature from 22 to 45 °C. Methane oxidation was measured at potential rates up to 141 μmol CH4 d(-1) g(-1) sediment. Active methanotrophs were identified using (13) CH4 stable-isotope probing (SIP) incubations performed at close to in situ temperatures for each site. Quantitative (q) PCR of pmoA genes identified the position of the heavy ((13) C-labelled) DNA fractions in density gradients… Expand
Anaerobic carboxydotrophic bacteria in geothermal springs identified using stable isotope probing
TLDR
The results show that bacteria capable of using CO as a carbon source are common in geothermal springs, and that thermophilic carboxydotrophs are probably already quite well known from cultivation studies. Expand
Acid-Tolerant Moderately Thermophilic Methanotrophs of the Class Gammaproteobacteria Isolated From Tropical Topsoil with Methane Seeps
TLDR
Strains BFH1 and BFH2 are, to the authors' knowledge, the first isolated acid-tolerant moderately thermophilic methane oxidizers of the class Gammaproteobacteria and each strain probably denotes a novel species and they most likely represent a novel genus within the family Methylococcaceae of type I methanotrophs. Expand
So close, so different: geothermal flux shapes divergent soil microbial communities at neighbouring sites.
TLDR
Next-Generation Sequencing techniques were applied to describe the bacterial and archaeal communities which have been linked to the physicochemical conditions and the geothermal sources of energy available at the two sites, finding that abundant availability of NH4(+) and other high energy electron donors and acceptors determined by the hydrothermal flux in this site create more energetically favourable conditions for chemolithotrophs that outcompete methanotrophic soils. Expand
The influence of temperature and pH on bacterial community composition of microbial mats in hot springs from Costa Rica
TLDR
Both pH and temperature were relevant for community composition even within the moderate ranges of variables studied, providing a basis for an understanding of the physicochemical influences in moderately thermophilic microbial mats. Expand
Identification of growing bacteria during litter decomposition in freshwater through H218O quantitative stable isotope probing.
TLDR
It is shown that a large proportion of the bacterial taxa found on decomposing leaf litter grew slowly, and several low abundance taxa were highly enriched, and that quantitative stable isotope probing with H218O can be used to advance understanding of microorganisms in freshwater by identifying species that are growing in complex communities. Expand
Thermophilic methanotrophs: In hot pursuit.
TLDR
It is asserted, on the basis of recently reported molecular and geochemical data, that geothermal ecosystems host hitherto unidentified species capable of methane oxidation at hotter temperatures. Expand
Phylogenetically distinct methanotrophs modulate methane oxidation in rice paddies across Taiwan
Abstract Rice paddies are considerable sources of methane because of the highly reduced soil oxidation–reduction conditions during rice cultivation. In this ecosystem, active methane-oxidizingExpand
Niche Differentiation of Active Methane-Oxidizing Bacteria in Estuarine Mangrove Forest Soils in Taiwan
TLDR
Genetic analysis and phylogenetic analysis suggest that Type I methanotrophs display niche differentiation associated with environmental differences between upstream and downstream mangrove soils of the Tamsui estuary in northwestern Taiwan. Expand
Community Structure of Active Aerobic Methanotrophs in Red Mangrove (Kandelia obovata) Soils Under Different Frequency of Tides
TLDR
Overall, this study showed the feasibility of 13CH4 DNA-SIP to study the active methanotrophic communities in mangrove forest soils and revealed differences in the methanOTrophic community structure between coastal mangroves experiencing different tide frequencies. Expand
Diversity and Distribution of Thermophilic Bacteria in Hot Springs of Pakistan
TLDR
A comprehensive, culture-independent survey of microbial communities in nine samples from these geothermal fields by employing a bar-coded pyrosequencing technique provided novel insights into the nature of ecological interactions among important taxa in these communities. Expand
...
1
2
3
...

References

SHOWING 1-10 OF 59 REFERENCES
Stable Isotope Probing Analysis of the Diversity and Activity of Methanotrophic Bacteria in Soils from the Canadian High Arctic
TLDR
New information is providing new information supporting the potential importance of Methylobacter spp. Expand
Identification of functionally active aerobic methanotrophs in sediments from an arctic lake using stable isotope probing.
TLDR
The results show that a diverse microbial consortium acquired carbon from CH(4) in the sediments of this arctic lake during ice-free summer conditions. Expand
Identification of the Functionally Active Methanotroph Population in a Peat Soil Microcosm by Stable-Isotope Probing
TLDR
The active population of low-affinity methanotrophs in a peat soil microcosm was characterized by stable-isotope probing and Sequences related to extant type I and type II methanOTrophs were identified, indicating that these methanots were active in peat exposed to 8% (vol/vol) CH4. Expand
Analysis of methanotrophic bacteria in Movile Cave by stable isotope probing.
TLDR
It is suggested that aerobic methanotrophs actively convert CH4 into complex organic compounds in Movile Cave and thus help to sustain a diverse community of microorganisms in this closed ecosystem. Expand
Identity of active methanotrophs in landfill cover soil as revealed by DNA-stable isotope probing.
TLDR
Environmental conditions in the slightly acidic peat soil from Roscommon landfill cover allow establishment of both Type I and Type II methanotrophs, closely related to the genera Methylocella and Methylocapsa and to Methylocystis species. Expand
Methanotrophic bacteria in oilsands tailings ponds of northern Alberta
TLDR
The SIP-PCR results demonstrated that the Methylocaldum and Methylomonas spp. Expand
Methane oxidation activity and diversity of aerobic methanotrophs in pH-neutral and semi-neutral thermal springs of the Kunashir Island, Russian Far East
TLDR
The results suggest that methanotrophs inhabiting thermal springs with temperature exceeding 50 °C may represent novel thermophilic and thermotolerant species of the genera Methylocystis and Methylothermus, as well as previously undescribed Gammaproteobacteria. Expand
Distribution and diversity of Verrucomicrobia methanotrophs in geothermal and acidic environments.
TLDR
There appears to be a thermophilicgroup and a mesophilic group of methanotrophic Verrucomicrobia, however, both were detected only in acidic geothermal environments. Expand
The active methanotrophic community in a wetland from the High Arctic.
TLDR
Results indicate that the extant M. tundripaludum is an active methane oxidizer at its locus typicus; furthermore, Methylobacter seems to be the dominant active genus. Expand
Diversity and activity of methanotrophs in alkaline soil from a Chinese coal mine.
TLDR
DNA sequence information on the most abundant, active methanotrophs in this alkaline soil will facilitate the design of oligonucleotide probes to monitor enrichment cultures when isolating key alkaliphilic methanOTrophs from such environments. Expand
...
1
2
3
4
5
...