Methanol-Induced Unfolding and Refolding of Cytochrome b5 and Its P40V Mutant Monitored by UV-Visible, CD, and Fluorescence Spectra

@article{Wang1999MethanolInducedUA,
  title={Methanol-Induced Unfolding and Refolding of Cytochrome b5 and Its P40V Mutant Monitored by UV-Visible, CD, and Fluorescence Spectra},
  author={Z. Q. Wang and Y. H. Wang and Wen Qian and H. H. Wang and Lijuan Chunyu and Y. Xie and Z. X. Huang},
  journal={Journal of Protein Chemistry},
  year={1999},
  volume={18},
  pages={547-555}
}
In order to illustrate the structural importance of proline-40 of cytochrome b5 (Cyt b5), the P40V mutant gene was constructed. Unfolding and refolding of Cyt b5 induced by methanol was investigated by means of the UV-visible spectrum, circular dichroism, and the fluorescence spectrum. Methanol denaturation of Cyt b5 is a cooperative process, that is, the heme group dissociates from the heme pocket accompanied by unfolding of the polypeptide chain both in the secondary and tertiary structures… 
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