Metastatic melanoma cell heparanase. Characterization of heparan sulfate degradation fragments produced by B16 melanoma endoglucuronidase.

@article{Nakajima1984MetastaticMC,
  title={Metastatic melanoma cell heparanase. Characterization of heparan sulfate degradation fragments produced by B16 melanoma endoglucuronidase.},
  author={Motowo Nakajima and Tatsuro Irimura and Nicola Di Ferrante and Garth L. Nicolson},
  journal={The Journal of biological chemistry},
  year={1984},
  volume={259 4},
  pages={2283-90}
}
Heparan sulfate (HS), a prominent component of vascular endothelial basal lamina, is cleaved into large Mr fragments and solubilized from subendothelial basal lamina-like matrix by metastatic murine B16 melanoma cells. We have examined the degradation products of HS and other purified glycosaminoglycans produced by B16 cells. Glycosaminoglycans 3H-labeled at their reducing termini or metabolically labeled with [35S]sulfate were incubated with B16 cell extracts in the absence or presence of D… CONTINUE READING