Metastatic burden in nude mice organs measured using prostate tumor PC-3 cells expressing the luciferase gene as a quantifiable tumor cell marker.

@article{Rubio2000MetastaticBI,
  title={Metastatic burden in nude mice organs measured using prostate tumor PC-3 cells expressing the luciferase gene as a quantifiable tumor cell marker.},
  author={N{\'u}ria Rubio and M M Villacampa and Nadia El Hilali and Jer{\'o}nimo Blanco},
  journal={The Prostate},
  year={2000},
  volume={44 2},
  pages={133-43}
}
BACKGROUND Sensitive procedures for quantitative measurement of tumor cell spread as a function of time and primary tumor size are necessary to generate models of metastasis and formulate therapies. METHODS Prostate carcinoma cells PC-3.luc expressing the luciferase gene were intramuscularly inoculated in nude mice to generate experimental tumors. Metastatic cells in target organs were easily counted by their capacity to produce light. RESULTS Tumor cells were very mobile and migrated to… CONTINUE READING