Metalloselenonein, the selenium analogue of metallothionein: synthesis and characterization of its complex with copper ions.

@article{Oikawa1991MetalloselenoneinTS,
  title={Metalloselenonein, the selenium analogue of metallothionein: synthesis and characterization of its complex with copper ions.},
  author={Tadao Oikawa and Nobuyoshi Esaki and Hiroyuki Tanaka and Kenji Soda},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 8},
  pages={3057-9}
}
We used an automated peptide synthesizer to produce a peptide, metalloselenonein, that contains selenocysteine residues substituted for all cysteine residues in Neurospora crassa copper metallothionein. Metalloselenonein binds 3 mol of Cu(I) per mol. This adduct shows a broad absorption band between 230 and 400 nm and a fluorescence band at 395 nm, which can be attributed to copper-selenolate coordination. The circular dichroism spectrum of the copper-metalloselenonein complex shows a positive… CONTINUE READING

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