Metal-mediated crystallization of the xylose transporter XylE from Escherichia coli in three different crystal forms.

  title={Metal-mediated crystallization of the xylose transporter XylE from Escherichia coli in three different crystal forms.},
  author={Esben Meldgaard Quistgaard and Christian Loew and Per Moberg and Paer Nordlund},
  journal={Journal of structural biology},
  volume={184 2},
XylE is a major facilitator (MFS) xylose transporter, which is homologous to the mammalian glucose transporters (GLUT family). We have previously reported the structure of XylE in fully inward open and partially occluded inward open conformations in space groups P61 and C2, respectively. Here we present the crystallization of a third crystal form, P212121 (~4 Å resolution), also representing an inward facing conformation, and analyze all three forms in terms of crystallization conditions and… CONTINUE READING

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