Metal-mediated crystallization of the xylose transporter XylE from Escherichia coli in three different crystal forms.

@article{Quistgaard2013MetalmediatedCO,
  title={Metal-mediated crystallization of the xylose transporter XylE from Escherichia coli in three different crystal forms.},
  author={Esben Meldgaard Quistgaard and Christian Loew and Per Moberg and Paer Nordlund},
  journal={Journal of structural biology},
  year={2013},
  volume={184 2},
  pages={375-8}
}
XylE is a major facilitator (MFS) xylose transporter, which is homologous to the mammalian glucose transporters (GLUT family). We have previously reported the structure of XylE in fully inward open and partially occluded inward open conformations in space groups P61 and C2, respectively. Here we present the crystallization of a third crystal form, P212121 (~4 Å resolution), also representing an inward facing conformation, and analyze all three forms in terms of crystallization conditions and… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

References

Publications referenced by this paper.
Showing 1-10 of 11 references

PHENIX: a comprehensive Python-based system for macromolecular structure solution

Acta crystallographica. Section D, Biological crystallography • 2010
View 3 Excerpts
Highly Influenced

An approach to crystallizing proteins by metal-mediated synthetic symmetrization.

Protein science : a publication of the Protein Society • 2011
View 1 Excerpt

Xds. Acta crystallographica

W. Kabsch
Acta Crystallogr. Sect. D: Biol. Crystallogr • 2010
View 1 Excerpt

Toward the structural genomics of complexes: crystal structure of a PE/PPE protein complex from Mycobacterium tuberculosis.

Proceedings of the National Academy of Sciences of the United States of America • 2006
View 1 Excerpt

The PyMol Molecular Graphics System

W. Delano
DeLano Scientific LLC, San Carlos, CA, USA. http://www.pymol.org. • 2003
View 1 Excerpt

Major facilitator superfamily.

Microbiology and molecular biology reviews : MMBR • 1998

Similar Papers

Loading similar papers…