Metal-ion-dependent catalysis and specificity of CCA-adding enzymes: a comparison of two classes.

@article{Hou2005MetaliondependentCA,
  title={Metal-ion-dependent catalysis and specificity of CCA-adding enzymes: a comparison of two classes.},
  author={Y M Hou and Shan-Qing Gu and Haiping Zhou and Lindsey A Ingerman},
  journal={Biochemistry},
  year={2005},
  volume={44 38},
  pages={12849-59}
}
The CCA-adding enzymes [ATP(CTP):tRNA nucleotidyl transferases] catalyze synthesis of the conserved and essential CCA sequence to the tRNA 3' end. These enzymes are divided into two classes of distinct structures that differ in the overall orientation of the head to tail domains. However, the catalytic core of the two classes is conserved and contains three carboxylates in a geometry commonly found in DNA and RNA polymerases that use the two-metal-ion mechanism for phosphoryl transfer. Two… CONTINUE READING