Metal ion catalysis in the Tetrahymena ribozyme reaction

  title={Metal ion catalysis in the Tetrahymena ribozyme reaction},
  author={J. Piccirilli and J. S. Vyle and M. Caruthers and T. Cech},
  • J. Piccirilli, J. S. Vyle, +1 author T. Cech
  • Published 1993
  • Chemistry, Medicine
  • Nature
  • ALL catalytic RNAs (ribozymes) require or are stimulated by divalent metal ions, but it has been difficult to separate the contribution of these metal ions to formation of the RNA tertiary structure1 from a more direct role in catalysis. The Tetrahymena ribozyme catalyses cleavage of exogenous RNA2,3 or DNA4,5 substrates with an absolute requirement for Mg2+ or Mn2+ (ref. 6). A DNA substrate, in which the bridging 3′ oxygen atom at the cleavage site is replaced by sulphur, is cleaved by the… CONTINUE READING
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