Metal chelate affinity chromatography, a new approach to protein fractionation

@article{Porath1975MetalCA,
  title={Metal chelate affinity chromatography, a new approach to protein fractionation},
  author={Jerker O. Porath and Jan Per Erik Carlsson and Ingmar Olsson and Greta Belfrage},
  journal={Nature},
  year={1975},
  volume={258},
  pages={598-599}
}
CONVENTIONAL nonspecific precipitation methods sometimes depend on affinities which can be used in a more selective fashion by modern chromatographic techniques. The affinity of proteins for heavy metal ions, for example, may provide a basis for their purification and analysis. A highly flexible method based on such affinities is described here. 
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