Metal chelate affinity chromatography, a new approach to protein fractionation

  title={Metal chelate affinity chromatography, a new approach to protein fractionation},
  author={Jerker O. Porath and Jan Per Erik Carlsson and Ingmar Olsson and Greta Belfrage},
CONVENTIONAL nonspecific precipitation methods sometimes depend on affinities which can be used in a more selective fashion by modern chromatographic techniques. The affinity of proteins for heavy metal ions, for example, may provide a basis for their purification and analysis. A highly flexible method based on such affinities is described here. 
Affinity chromatography of phosphorylated proteins.
This chapter covers the use of immobilized metal ion affinity chromatography (IMAC) for enrichment of phosphorylated proteins. Some requirements for successful enrichment of these types of proteinsExpand
Metal chelate affinity precipitation: a new approach to protein purification
Metal chelate affinity precipitation of proteins, a method combining metal–protein interaction and affinity precipitation is being discussed as a selective separation process for proteins. TheExpand
Affinity precipitation of proteins using metal chelates.
Copolymers of vinylimidazole with N-isopropylacrylamide are easily synthesized by radical polymerization and are capable of selectively precipitating proteins with natural metal-binding groups or histidine-tagged recombinant proteins. Expand
Use of metal-chelate affinity chromatography and hydrophobic interaction chromatography for purification of placental protein 12.
Placental protein 12 was isolated from amniotic fluid by metal-chelate affinity chromatography and hydrophobic interaction chromatography under mild conditions for desorption, resulting in large quantities of a highly purified preparation in one run. Expand
Pseudobiospecific ligand affinity chromatography
Abstract Pseudobiospecific ligand affinity chromatography is based on the use of robust and inexpensive substitutes for the biological ligands used in biospecific ligand affinity chromatography. TheExpand
LIQUID CHROMATOGRAPHY | Affinity Chromatography
Affinity chromatography is a liquid chromatographic method in which a biological agent or biomimetic ligand is used for the selective retention of complementary compounds. This article presents anExpand
Novel separations based on affinity interactions
Novel purification processes have been developed, based on the affinity interaction between complementary molecules, to circumvent the difficulties associated with affinity chromatography. DependingExpand
Preparative affinity chromatography of proteins
Abstract This review describes the role of affinity chromatography in large-scale protein purification. The currently available affinity supports are examined with respect to their use in theExpand
Affinity Chromatography And Metal Chelate Affinity Chromatography
The extremely rapid growth in the field of protein chemistry during the past 50 years has been due in large part to the development of very effective techniques of protein separation and analysis. AExpand
Study on the affinity characteristics of proteins on the immobilized metal affinity chromatography column
Study on affinity characteristic paramaters (KPML & ΛPML) of proteins on the iminodisuccinic acid metal chelate copper column by frontal chromatography.


Preparation of adsorbents for biospecific affinity chromatography. Attachment of group-containing ligands to insoluble polymers by means of bifuctional oxiranes.
Fractionation of different forms of trypsin on soya beantrypsin inhibitor agarose is described in order to illustrate the use of oxirane-agarose in biospecific affinity chromatography. Expand
The purification of yeast enolase by zone electrophoresis and ion-exchange chromatography, and the existence of several active forms of the enzyme
Abstract 1. 1. A new method for the purification of yeast enolase has been described. The procedure involves zone electrophoresis in cellulose columns, and specific adsorption of the enzyme on the MgExpand