Metal Ions Play an Essential Catalytic Role in the Mechanism of Ketol-Acid Reductoisomerase.

@article{Tadrowski2016MetalIP,
  title={Metal Ions Play an Essential Catalytic Role in the Mechanism of Ketol-Acid Reductoisomerase.},
  author={Sonya Tadrowski and Marcelo Monteiro Pedroso and Volker Sieber and James A. Larrabee and Luke W. Guddat and Gerhard Schenk},
  journal={Chemistry},
  year={2016},
  volume={22 22},
  pages={7427-36}
}
Ketol-acid reductoisomerase (KARI) is a Mg(2+) -dependent enzyme in the branched-chain amino acid biosynthesis pathway. It catalyses a complex two-part reaction: an alkyl migration followed by a NADPH-dependent reduction. Both reactions occur within the one active site, but in particular, the mechanism of the isomerisation step is poorly understood. Here… CONTINUE READING