Metal Binding Properties of a Monoclonal Antibody Directed toward Metal-Chelate Complexes*

  title={Metal Binding Properties of a Monoclonal Antibody Directed toward Metal-Chelate Complexes*},
  author={Diane A. Blake and Pampa Chakrabarti and Mehraban Khosraviani and Frank M. Hatcher and Connie M Westhoff and Peter Goebel and Dwane E. Wylie and Robert C. Blake},
  journal={The Journal of Biological Chemistry},
  pages={27677 - 27685}
A monoclonal antibody that recognizes cadmium-EDTA complexes has been produced by the injection of BALB/c mice with a metal-chelate complex covalently coupled to a carrier protein. The ability of purified antibody to recognize 16 different metal-EDTA complexes was assessed by measuring equilibrium binding constants using a KinExA™ immunoassay instrument. The antibody bound to cadmium- and mercury-EDTA complexes with equilibrium dissociation constants of 21 and 26 nM, respectively. All other… 
Binding properties of a monoclonal antibody directed toward lead-chelate complexes.
A monoclonal antibody (2C12) that recognizes a Pb(II)-cyclohexyldiethylenetriamine pentaacetic acid complex was produced by the injection of BALB/c mice with a P b( II)-chelate complex covalently coupled to a carrier protein by measuring equilibrium dissociation constants.
Allosteric binding properties of a monoclonal antibody and its Fab fragment.
These data are interpreted in terms of a model in which aminobenzyl-DTPA and its complexes bind both to the antigen binding site and to multiple charged sites on the surface of the compact immunoglobulin; and the bound, highly charged ligands interact in a complicated fashion through the apolar core of the folded antibody.
Antibody-based sensors for heavy metal ions.
The KinExA 3000 instrument and the Cd(II)-specific antibody were used to construct a prototype assay that could correctly assess the concentration of cadmium spiked into a groundwater sample.
Preparation of Novel Monoclonal Antibodies Against Chelated Cadmium Ions
Spike–recovery studies in tap water showed that the antibody A3 could be used for cadmium detection in drinking water and a cheaper bifunctional chelator was proposed, 1-(4-aminobenzyl) ethylenediamine N,N, N′,N′-tetraacetic acid (aminOBenzyl-EDTA).
Preparation of Specific Monoclonal Antibodies Against Chelated Copper Ions
Copper ions are too small to elicit an immune response. Therefore, copper was conjugated to carrier proteins using S-2-(4-isothiocyanatobenzyl)-1, 4, 7, 10-tetraazacyclododecane-1, 4, 7,
Detection of copper ions using microcantilever immunosensors and enzyme-linked immunosorbent assay.
A sensitive and specific monoclonal antibody (designated as mAb6A9) recognizing a Cu(II)-ethylenediamine-N,N,N',N'-tetraacetic acid (EDTA) complex but not metal-free EDTA was obtained by using an
Preparation of Anti-Cadmium-EDTA Complex Polyclonal Antibody and Its Application for Determination of Cadmium in Aqueous Solution
Abstract A polyclonal antibody that can recognize cadmium-ethylenediaminetetraacetic acid (CD-EDTA) complex was prepared via the injection of New Zealand white rabbits with
A fluorescence polarization immunoassay for cadmium(II)
Abstract A target for antibody production was prepared by a 1 : 1 reaction of the bicyclic dianhydride of diethylenetriamine-N,N,N′,N′′,N′′-pentaacetic acid (DTPA) with
Development of ELISA for Detection of Mercury Based on Specific Monoclonal Antibodies Against Mercury-Chelate
Results indicate that this method could be used for monitoring mercury of water and correlated well with graphite furnace atomic absorption spectrometry (GFAAS), and the mean recovery was 88.82% to 104.64%.
Characterization of a new monoclonal antibody against mercury (II)
Monoclonal antibodies (mabs) were produced against mercury (II) and an enzyme immunoassay was developed for the detection of mercury (II) in water. Since mercury (II) ions are too small to elicit an


How the anti-(metal chelate) antibody CHA255 is specific for the metal ion of its antigen: X-ray structures for two Fab'/hapten complexes with different metals in the chelate.
: Antibodies with bound metal-chelate haptens provide new means for exploiting the diverse properties of metallic elements. The murine monoclonal antibody CHA255 (IgG1 lambda) binds the metal-chelate
Monoclonal antibodies specific for mercuric ions.
  • D. Wylie, D. Lu, +4 authors F. Wagner
  • Chemistry, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1992
The successful induction of antibodies that react very specifically with mercuric ions in solution regardless of the presence of a carrier is demonstrated, demonstrating that the antibodies were capable of binding to mercural ions in the presenceof a different counterion than the one used in the immunogen.
Antibodies against metal chelates
These antibodies show a remarkable preference for indium chelates; changing to another metal such as scandium or gallium can decrease the antibody-binding constant by more than three orders of magnitude, which introduces a new degree of control over the biological distributions of chelated radionuclides.
Enzyme immunoassay to determine heavy metals using antibodies to specific metal-EDTA complexes: optimization and validation of an immunoassay for soluble indium.
An immunoassay that measures soluble indium at concentrations from 0.005 ppb to 320 ppm is described, utilized a monoclonal antibody that binds specifically to indium-EDTA complexes in an antigen-inhibition format and correlated closely with the values obtained using atomic absorption spectroscopy.
Development and characterization of monoclonal antibodies with specificity for metallic radioisotope chelators linked to antibodies and other proteins.
Monoclonal antibodies with specificity for the diethylenetriaminepentaacetic acid (DTPA) portion of the GYK-DTPA (glycyl-tyrosyl-lysine- DTPA) linker structure used to chelate metals to immunoglobulins have been prepared and demonstrated that DTPA, rather than GYk, was the binding site of the antibodies tested.
Nature of the bifunctional chelating agent used for radioimmunotherapy with yttrium-90 monoclonal antibodies: critical factors in determining in vivo survival and organ toxicity.
Four chelating agents emerged as promising immunotherapeutic reagents: isothiocyanate benzyl DTPA and its derivatives 1B3M, MX, and 1M3B were very stable in vivo and suitable for yttrium-monoclonal antibody radioimmunotherapy.
Elicitation of a predominantly lambda light chain-bearing antibody response in BALB/c mice to a novel bifunctional aminocarboxylate chelating agent.
A panel of murine monoclonal antibodies has been raised against the aminocarboxylate bifunctional chelating agent PA-DOTA complexed with Samarium and the binding specificity of 11 of the antibodies was examined by competition assays.
Detection of mercuric ions in water by ELISA with a mercury-specific antibody.
An immunoassay that detects mercuric ions in water at concentrations of 0.5 ppb and above is described, which is as sensitive as cold-vapor atomic absorption for mercury detection and can be performed with only 100 microliters of sample.
Molecular design to mimic the copper(II) transport site of human albumin: studies of equilibria between copper(II) and glycylglycyl-L-histidine-N-methyl amide and comparison with human albumin
In continuing the investigation of designing the specific Cu(II)-transport site of human serum albumin, the peptide derivative glycylglycyl-L-histidine-N-methyl amide was designed to approximate more
Kinetics of the dissociation of indium-(p-substituted-benzyl)ethylenediaminetetraacetic acid hapten analogues from the monoclonal anti-hapten antibody CHA255.
The dissociation rate constant was found to be an extremely sensitive indicator of the hapten-antibody interaction that can be measured very precisely and results from distinct conformations of the complex dissociating at different rates.