Metal Binding Properties of a Monoclonal Antibody Directed toward Metal-Chelate Complexes*

@article{Blake1996MetalBP,
  title={Metal Binding Properties of a Monoclonal Antibody Directed toward Metal-Chelate Complexes*},
  author={Diane A. Blake and Pampa Chakrabarti and Mehraban Khosraviani and Frank M. Hatcher and Connie M Westhoff and Peter Goebel and Dwane E. Wylie and Robert C. Blake},
  journal={The Journal of Biological Chemistry},
  year={1996},
  volume={271},
  pages={27677 - 27685}
}
A monoclonal antibody that recognizes cadmium-EDTA complexes has been produced by the injection of BALB/c mice with a metal-chelate complex covalently coupled to a carrier protein. The ability of purified antibody to recognize 16 different metal-EDTA complexes was assessed by measuring equilibrium binding constants using a KinExA™ immunoassay instrument. The antibody bound to cadmium- and mercury-EDTA complexes with equilibrium dissociation constants of 21 and 26 nM, respectively. All other… 
Binding properties of a monoclonal antibody directed toward lead-chelate complexes.
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Allosteric binding properties of a monoclonal antibody and its Fab fragment.
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These data are interpreted in terms of a model in which aminobenzyl-DTPA and its complexes bind both to the antigen binding site and to multiple charged sites on the surface of the compact immunoglobulin; and the bound, highly charged ligands interact in a complicated fashion through the apolar core of the folded antibody.
Antibody-based sensors for heavy metal ions.
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The KinExA 3000 instrument and the Cd(II)-specific antibody were used to construct a prototype assay that could correctly assess the concentration of cadmium spiked into a groundwater sample.
Preparation of Novel Monoclonal Antibodies Against Chelated Cadmium Ions
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Spike–recovery studies in tap water showed that the antibody A3 could be used for cadmium detection in drinking water and a cheaper bifunctional chelator was proposed, 1-(4-aminobenzyl) ethylenediamine N,N, N′,N′-tetraacetic acid (aminOBenzyl-EDTA).
Preparation of Specific Monoclonal Antibodies Against Chelated Copper Ions
Copper ions are too small to elicit an immune response. Therefore, copper was conjugated to carrier proteins using S-2-(4-isothiocyanatobenzyl)-1, 4, 7, 10-tetraazacyclododecane-1, 4, 7,
Detection of copper ions using microcantilever immunosensors and enzyme-linked immunosorbent assay.
A sensitive and specific monoclonal antibody (designated as mAb6A9) recognizing a Cu(II)-ethylenediamine-N,N,N',N'-tetraacetic acid (EDTA) complex but not metal-free EDTA was obtained by using an
Preparation of Anti-Cadmium-EDTA Complex Polyclonal Antibody and Its Application for Determination of Cadmium in Aqueous Solution
Abstract A polyclonal antibody that can recognize cadmium-ethylenediaminetetraacetic acid (CD-EDTA) complex was prepared via the injection of New Zealand white rabbits with
A fluorescence polarization immunoassay for cadmium(II)
Abstract A target for antibody production was prepared by a 1 : 1 reaction of the bicyclic dianhydride of diethylenetriamine-N,N,N′,N′′,N′′-pentaacetic acid (DTPA) with
Development of ELISA for Detection of Mercury Based on Specific Monoclonal Antibodies Against Mercury-Chelate
TLDR
Results indicate that this method could be used for monitoring mercury of water and correlated well with graphite furnace atomic absorption spectrometry (GFAAS), and the mean recovery was 88.82% to 104.64%.
Characterization of a new monoclonal antibody against mercury (II)
Monoclonal antibodies (mabs) were produced against mercury (II) and an enzyme immunoassay was developed for the detection of mercury (II) in water. Since mercury (II) ions are too small to elicit an
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TLDR
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