Metagenomics to Paleogenomics: Large-Scale Sequencing of Mammoth DNA

  title={Metagenomics to Paleogenomics: Large-Scale Sequencing of Mammoth DNA},
  author={Hendrik N. Poinar and Carsten Schwarz and Ji Qi and Beth Shapiro and Ross D.E. Macphee and Bernard Buigues and Alexei Tikhonov and Daniel H. Huson and Lynn P. Tomsho and Alexander F. Auch and Markus Rampp and Webb Miller and Stephan C. Schuster},
  pages={392 - 394}
We sequenced 28 million base pairs of DNA in a metagenomics approach, using a woolly mammoth (Mammuthus primigenius) sample from Siberia. As a result of exceptional sample preservation and the use of a recently developed emulsion polymerase chain reaction and pyrosequencing technique, 13 million base pairs (45.4%) of the sequencing reads were identified as mammoth DNA. Sequence identity between our data and African elephant (Loxodonta africana) was 98.55%, consistent with a paleontologically… 

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