Metagenomics for Mining New Genetic Resources of Microbial Communities

@article{Ferrer2008MetagenomicsFM,
  title={Metagenomics for Mining New Genetic Resources of Microbial Communities},
  author={Manuel Ferrer and Ana Beloqui and Kenneth N. Timmis and Peter N. Golyshin},
  journal={Journal of Molecular Microbiology and Biotechnology},
  year={2008},
  volume={16},
  pages={109 - 123}
}
Recent progress has revealed that the capture of genetic resources of complex microbial communities in metagenome libraries allows the discovery of a richness of new enzymatic diversity that had not previously been imagined. Activity-based screening of such libraries has demonstrated that this new diversity is not simply variations on known sequence themes, but rather the existence of entirely new sequence classes and novel functionalities. This new diversity, the surface of which has thus far… 
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References

SHOWING 1-10 OF 142 REFERENCES
Metagenomics: Application of Genomics to Uncultured Microorganisms
  • J. Handelsman
  • Biology
    Microbiology and Molecular Biology Reviews
  • 2004
TLDR
Reassembly of multiple genomes has provided insight into energy and nutrient cycling within the community, genome structure, gene function, population genetics and microheterogeneity, and lateral gene transfer among members of an uncultured community.
Metagenomic gene discovery: past, present and future.
Targeted Access to the Genomes of Low-Abundance Organisms in Complex Microbial Communities
TLDR
Genomic reconstruction from targeted cells of uncultured organisms isolated directly from the environment represents a powerful approach to access any specific members of a community and an alternative way to assess the community's metabolic potential.
Metagenomics: advances in ecology and biotechnology.
Metagenomics: genomic analysis of microbial communities.
TLDR
Progress toward understanding the biology of uncultured Bacteria, Archaea, and viruses through metagenomic analyses is described.
Metagenomics--the key to the uncultured microbes.
An application of statistics to comparative metagenomics
TLDR
A statistical method is used to compare curated subsystems, to predict the physiology, metabolism, and ecology from metagenomes, and reveals those subsystems that are more, or less, represented in the different environments that are compared.
Exploring the microbial biodegradation and biotransformation gene pool.
Metagenomic marine nitrogen fixation--feast or famine?
A molecular view of microbial diversity and the biosphere.
Over three decades of molecular-phylogenetic studies, researchers have compiled an increasingly robust map of evolutionary diversification showing that the main diversity of life is microbial,…
...
...