Metagenomic approach for the isolation of a novel low-temperature-active lipase from uncultured bacteria of marine sediment.

@article{Hrdeman2007MetagenomicAF,
  title={Metagenomic approach for the isolation of a novel low-temperature-active lipase from uncultured bacteria of marine sediment.},
  author={Fredrik H{\aa}rdeman and Sara Sj{\"o}ling},
  journal={FEMS microbiology ecology},
  year={2007},
  volume={59 2},
  pages={
          524-34
        }
}
A novel lipase was isolated from a metagenomic library of Baltic Sea sediment bacteria. Prokaryotic DNA was extracted and cloned into a copy control fosmid vector (pCC1FOS) generating a library of >7000 clones with inserts of 24-39 kb. Screening for clones expressing lipolytic activity based on the hydrolysis of tributyrin and p-nitrophenyl esters, identified 1% of the fosmids as positive. An insert of 29 kb was fragmented and subcloned. Subclones with lipolytic activity were sequenced and an… CONTINUE READING
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