Metagenomic analyses of viruses in stool samples from children with acute flaccid paralysis.

@article{Victoria2009MetagenomicAO,
  title={Metagenomic analyses of viruses in stool samples from children with acute flaccid paralysis.},
  author={Joseph G. Victoria and Amit Kapoor and Linlin Li and O. V. Blinkova and Beth Slikas and Chunlin Wang and Asif Naeem and Syed Sohail Zahoor Zaidi and Eric L Delwart},
  journal={Journal of virology},
  year={2009},
  volume={83 9},
  pages={4642-51}
}
We analyzed viral nucleic acids in stool samples collected from 35 South Asian children with nonpolio acute flaccid paralysis (AFP). Sequence-independent reverse transcription and PCR amplification of capsid-protected, nuclease-resistant viral nucleic acids were followed by DNA sequencing and sequence similarity searches. Limited Sanger sequencing (35 to 240 subclones per sample) identified an average of 1.4 distinct eukaryotic viruses per sample, while pyrosequencing yielded 2.6 viruses per… CONTINUE READING
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A highly diversified and prevalent new Picornaviridae genus in the stool of South Asian children

  • A. Kapoor, J. G. Victoria, +11 authors E. L. Delwart
  • Proc. Natl. Acad. Sci. USA
  • 2008

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