Metagenome analysis of Russian and Georgian Pyophage cocktails and a placebo‐controlled safety trial of single phage versus phage cocktail in healthy Staphylococcus aureus carriers

@article{McCallin2018MetagenomeAO,
  title={Metagenome analysis of Russian and Georgian Pyophage cocktails and a placebo‐controlled safety trial of single phage versus phage cocktail in healthy Staphylococcus aureus carriers},
  author={Shawna McCallin and Shafiqul Alam Sarker and Shamima Sultana and Frank Oechslin and Harald Br{\"u}ssow},
  journal={Environmental Microbiology},
  year={2018},
  volume={20},
  pages={3278–3293}
}
Bacteriophage therapy is a commonly used treatment for Staphylococcus aureus infections in countries of the former Soviet Union, using both single phages and phage cocktails. The scarce data available on Eastern phage cocktails prompted an investigation into commercially-available Pyophage cocktails from two different manufacturers used to treat skin and wound infections. Comparison of the metagenomic composition of two Pyophage products from Georgia and Russia revealed substantial differences… 

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References

SHOWING 1-10 OF 53 REFERENCES

Metagenomic Analysis of Therapeutic PYO Phage Cocktails from 1997 to 2014

TLDR
Next generation sequencing and metagenomics analysis was able to characterize and compare the content of PYO cocktails separated by 17 years in time, finding it to remain relatively stable over the years.

Characterization of vB_SauM-fRuSau02, a Twort-Like Bacteriophage Isolated from a Therapeutic Phage Cocktail

TLDR
It is concluded that fRuSau02, a phage therapy agent in Russia, can serve as an alternative to antibiotic therapy against S. aureus.

Characterization and complete genome of the virulent Myoviridae phage JD007 active against a variety of Staphylococcus aureus isolates from different hospitals in Shanghai, China

TLDR
Staphylococcus phage JD007 can potentially be used in phage therapy or for the detection of S. aureus through lytic and inhibition assays, and no identifiable resistance or virulence genes were found in the JD007 genome.

Improving the Safety of Staphylococcus aureus Polyvalent Phages by Their Production on a Staphylococcus xylosus Strain

TLDR
The identification of phage-insensitive strains underlines the importance of designing phage cocktails with broadly varying and overlapping host ranges and suggests that some staphylococcal phages can be propagated on food-grade bacteria for biocontrol and safety purposes.

Combined Use of Bacteriophage K and a Novel Bacteriophage To Reduce Staphylococcus aureus Biofilm Formation

TLDR
A novel phage, DRA88, was isolated and mixed with phage K to produce a high-titer mixture that showed strong lytic activity against a wide range of S. aureus isolates, including representatives of the major international MRSA clones and coagulase-negative Staphylococcus.

What Can We Learn from a Metagenomic Analysis of a Georgian Bacteriophage Cocktail?

TLDR
The Intesti phage cocktail, a key commercial product of the Eliava Institute, Georgia, has been tested on a selection of bacterial strains, sequenced as a metagenomic sample, de novo assembled and analyzed by bioinformatics methods.

Evaluation of lytic activity of staphylococcal bacteriophage Sb‐1 against freshly isolated clinical pathogens

TLDR
The sequence analyses of phage genome showed absence of bacterial virulence genes, and this phage still reveals a high spectrum of lytic activity in vitro against freshly isolated, genetically different clinical samples obtained from the local hospitals, as well as the clinics from different geographical areas.

Potential of the Polyvalent Anti-Staphylococcus Bacteriophage K for Control of Antibiotic-Resistant Staphylococci from Hospitals

TLDR
In in vitro inhibitory assays, a lytic bacteriophage was assessed in vitro for its ability to inhibit emerging drug-resistant Staphylococcus aureus strains from hospitals and other species of Staphyllococcus isolated from bovine infections, enforcing the principle that, while certain target bacteria may be relatively insensitive to lytic phage, this can be overcome by obtaining modified phage variants from passage of the phage through the insensitive strains.

Phages and the Evolution of Bacterial Pathogens: from Genomic Rearrangements to Lysogenic Conversion

TLDR
The current review presents the available genomics and biological data on prophages from bacterial pathogens in an evolutionary framework to demonstrate that the chromosomes from bacteria and their viruses (bacteriophages) are coevolving.
...