Metabolomics for the characterization of cytochromes P450-dependent fatty acid hydroxylation reactions in Arabidopsis

@article{Kai2009MetabolomicsFT,
  title={Metabolomics for the characterization of cytochromes P450-dependent fatty acid hydroxylation reactions in Arabidopsis},
  author={Kosuke Kai and Hiroko Hashidzume and Kazuya Yoshimura and Hideyuki Suzuki and Nozomu Sakurai and Daisuke Shibata and Daisaku Ohta},
  journal={Plant Biotechnology},
  year={2009},
  volume={26},
  pages={175-182}
}
Genome sequence analysis has revealed the presence of almost infinite numbers of cytochrome P450 genes in a variety of organisms. To establish a robust experimental platform from which to explore the catalytic potential of those putative P450 proteins, we have developed a comprehensive metabolic profiling system based on Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR/MS) analysis, together with recombinant enzyme studies. Here, we report the enzymatic properties of CYP78A5… 

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