Metabolites and some characteristics of the metabolism of 3 H-digoxigenin by rat liver homogenates.

@article{Talcott1972MetabolitesAS,
  title={Metabolites and some characteristics of the metabolism of 3 H-digoxigenin by rat liver homogenates.},
  author={R. Talcott and S. Stohs and M. El-Olemy},
  journal={Biochemical pharmacology},
  year={1972},
  volume={21 15},
  pages={
          2001-6
        }
}
Abstract 3 H-digoxigenin is metabolized by rat liver homogenates to 3 H-3-epidigoxigenin and 3 H-3-dehydrodigoxigenin. Maximum conversion occurs after approximately 80 min of incubation. The amount of each metabolite which accumulates depends on the amount of NADP + added to the incubation mixture. No formation of more polar metabolites was observed, indicating that a microsomal hydroxylating system may not be operative in the metabolism of digoxigenin. 
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References

SHOWING 1-5 OF 5 REFERENCES
Metabolism in vitro of digitoxigenin by rat liver homogenates.
TLDR
Digitoxigenin is metabolized in vitro by rat liver homogenates and subcellular fractions and the microsomal hydroxylation system can be induced by treatment of the animals with phenobarbital. Expand
The clinical pharmacology of digitalis glycosides: a review.
TLDR
The metabolic half-times and excretion rates of labeled cardiac glycosides are related to pharmacologic or therapeutic activity and are related primarily to digoxin excretio. Expand
Effect of spironolactone and norbolethone on the toxicity of digitalis compounds in the rat
TLDR
It appears that the ability to antagonize the toxicity of digitalis compounds represents a pharmacological property independent of all known steroid hormone actions, since spironolactone is virtually devoid of these. Expand
Umwandlung von Cardenoliden durch Mikroorganismen III. Umsetzung von Aglykonen und Glykosiden mit Fusarium lini (BOLLEY). 4. Mitteilung über Reaktionen mit Mikroorganismen
Das Verhalten verschiedener digitaloider Aglykone und Glykoside gegenuber Kulturen von Fusarium lini (BOLLEY) wurde untersucht.