Metabolite profiling of Chlamydomonas reinhardtii under nutrient deprivation.

@article{Blling2005MetabolitePO,
  title={Metabolite profiling of Chlamydomonas reinhardtii under nutrient deprivation.},
  author={Christian B{\"o}lling and Oliver Fiehn},
  journal={Plant physiology},
  year={2005},
  volume={139 4},
  pages={
          1995-2005
        }
}
A metabolite profiling technique for Chlamydomonas reinhardtii cells for multiparallel analysis of low-molecular weight polar compounds was developed. The experimental protocol was optimized to quickly inactivate enzymatic activity, achieve maximum extraction capacity, and process large sample quantities. As a result of the rapid sampling, extraction, and analysis by gas chromatography coupled to time-of-flight mass spectrometry, more than 800 analytes from a single sample could be measured, of… CONTINUE READING

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