Metabolism of the macrolide immunosuppressant, tacrolimus, by the pig gut mucosa in the Ussing chamber.

@article{Lampen1996MetabolismOT,
  title={Metabolism of the macrolide immunosuppressant, tacrolimus, by the pig gut mucosa in the Ussing chamber.},
  author={A. Lampen and Uwe Christians and A. K. Gonschior and Augustinus Bader and Ingelore Hackbarth and Wolfgang von Engelhardt and K. F. Sewing},
  journal={British journal of pharmacology},
  year={1996},
  volume={117 8},
  pages={1730-4}
}
1. The macrolide tacrolimus (FK506), used as an immunosuppressant, is a cytochrome P450 (CYP) 3A substrate in the liver. The metabolism of tacrolimus and the transport of its metabolites in the pig gut was studied in the Ussing chamber. Tacrolimus and its metabolites were quantified by h.p.l.c./mass spectrometry. 2. In the Ussing chamber, demethyl, didemethyl, hydroxy and hydroxy-demethyl tacrolimus were generated. Their formation was concentration- and time-dependent. The metabolite pattern… CONTINUE READING