Metabolism of rutin and poncirin by human intestinal microbiota and cloning of their metabolizing α-L-rhamnosidase from Bifidobacterium dentium.

  title={Metabolism of rutin and poncirin by human intestinal microbiota and cloning of their metabolizing $\alpha$-L-rhamnosidase from Bifidobacterium dentium.},
  author={Seo-Hyeon Bang and Yang-Jin Hyun and Ju-Sun Shim and Sung-Woon Hong and Dong-Hyun Kim},
  journal={Journal of microbiology and biotechnology},
  volume={25 1},
To understand the metabolism of flavonoid rhamnoglycosides by human intestinal microbiota, we measured the metabolic activity of rutin and poncirin (distributed in many functional foods and herbal medicine) by 100 human stool specimens. The average α-Lrhamnosidase activities on the p-nitrophenyl-α-L-rhamnopyranoside, rutin, and poncirin subtrates were 0.10 ± 0.07, 0.25 ± 0.08, and 0.15 ± 0.09 pmol/min/mg, respectively. To investigate the enzymatic properties, α-L-rhamnosidase-producing bacteria… 

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