Metabolism of mono- and dichloro-dibenzo-p-dioxins by Phanerochaete chrysosporium cytochromes P450

@article{Kasai2009MetabolismOM,
  title={Metabolism of mono- and dichloro-dibenzo-p-dioxins by Phanerochaete chrysosporium cytochromes P450},
  author={Noriyuki Kasai and Shin-ichi Ikushiro and Raku Shinkyo and Kaori Yasuda and Shinji Hirosue and Akira Arisawa and Hirofumi Ichinose and Hiroyuki Wariishi and Toshiyuki Sakaki},
  journal={Applied Microbiology and Biotechnology},
  year={2009},
  volume={86},
  pages={773-780}
}
The white-rot fungus Phanerochaete chrysosporium possesses biodegradative capabilities of polychlorinated dibenzo-p-dioxins (PCDDs). One hundred twenty yeast clones expressing individual P450s of P. chrysosporum (PcCYPs), generated in our previous efforts, were screened for transformation of dioxin, and 40 positive clones were obtained. Of these clones, six clones showed metabolism of 2-chloro-dibenzo-p-dioxin, and a microsomal PcCYP designated as PcCYP11a3 showed much higher activity than any… CONTINUE READING