Metabolism of glutamine and glutathione via gamma-glutamyltranspeptidase and glutamate transport in Helicobacter pylori: possible significance in the pathophysiology of the organism.

@article{Shibayama2007MetabolismOG,
  title={Metabolism of glutamine and glutathione via gamma-glutamyltranspeptidase and glutamate transport in Helicobacter pylori: possible significance in the pathophysiology of the organism.},
  author={Keigo Shibayama and Jun-ichi Wachino and Yoshichika Arakawa and Massoud Saidijam and Nicholas G. Rutherford and Peter J F Henderson},
  journal={Molecular microbiology},
  year={2007},
  volume={64 2},
  pages={396-406}
}
gamma-Glutamyltranspeptidase (GGT) is a periplasmic enzyme of Helicobacter pylori implicated in its pathogenesis towards mammalian cells. We have cloned and expressed the H. pylori strain 26695 recombinant GGT protein in Escherichia coli and purified it to homogeneity. The purified protein exhibited hydrolysis activity with very high affinities for glutamine and glutathione shown by apparent K(m) values lower than 1 muM. H. pylori cells were unable to take up extracellular glutamine and… CONTINUE READING

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