Metabolism of d-Arabinose: a New Pathway in Escherichia coli

@article{Leblanc1971MetabolismOD,
  title={Metabolism of d-Arabinose: a New Pathway in Escherichia coli},
  author={Donald J. Leblanc and Robert P. Mortlock},
  journal={Journal of Bacteriology},
  year={1971},
  volume={106},
  pages={90 - 96}
}
Several growth characteristics of Escherichia coli K-12 suggest that growth on l-fucose results in the synthesis of all the enzymes necessary for growth on d-arabinose. Conversely, when a mutant of E. coli is grown on d-arabinose, all of the enzymes necessary for immediate growth on l-fucose are present. Three enzymes of the l-fucose pathway in E. coli, l-fucose isomerase, l-fuculokinase, and l-fuculose-l-phospháte aldolase possess activity on d-arabinose, d-ribulose, and d-ribulose-l-phosphate… 
D-arabinose metabolism in Escherichia coli B: induction and cotransductional mapping of the L-fucose-D-arabinose pathway enzymes
TLDR
It was found that L-fucose and D-arabinose acted as the apparent inducers of the enzymes needed for their degradation, and intermediates did not act as inducers in E. coli B.
Construction of an improved D-arabinose pathway in Escherichia coli K-12
TLDR
Competition studies in chemostats showed that ribitol-positive strains, with a selection coefficient of 9%/h, have a significant competitive advantage over ribolt-negative strains.
Cross-induction of the L-fucose system by L-rhamnose in Escherichia coli
TLDR
It was shown that anaerobic growth on rhamnose induces expression of not only the fucO gene but also the entire fuc regulon, which is necessary for cross-induction of the L-fucose enzymes by rhamNose.
Constitutive activation of L-fucose genes by an unlinked mutation in Escherichia coli
TLDR
It is shown in this study that the noninducibility of the three proteins can be changed by two different kinds of suppressor mutations: one mapping external to and the other within the fuc gene cluster.
Regulation of the L-arabinose catabolic pathway in Aerobacter aerogenes.
Evolution of l-1,2-Propanediol Catabolism in Escherichia coli by Recruitment of Enzymes for l-Fucose and l-Lactate Metabolism
TLDR
It is shown that phage P1 cotransduces the genetic locus conferring this property and the genes for the utilization of l-fucose and an enzyme catalyzing formation of a reduced fermentation product anaerobically in wild-type cells functions aerobically to oxidize this same product in the mutant.
Ribulokinase Activity in Escherichia colil
TLDR
The behavior of the kinase product in the cysteine-carbazole and orcinol reactions, as well as the results of periodate oxidation assays, provided evidence that it was not D-ribulose-5-phosphate, and suggest that the natural substrate for this enzyme is L-fuculose.
Loss of aldehyde dehydrogenase in an Escherichia coli mutant selected for growth on the rare sugar L-galactose
TLDR
Complete aerobic utilization of carbons 4 through 6 of L-fucose depends not only on an adequate activity of aldehyde dehydrogenase to trap L-lactaldehyde as its anionic acid but also on the lack of L -1,2-propanediol oxidoreductase activity, which converts L- lactaldehyde to a readily excreted alcohol.
Loss of Aldehyde Dehydrogenase in an Escherichia coli Mutant Selected for Growth on the Rare Sugar L-Galactose
TLDR
A second mutation that abolished aldehyde dehydrogenase activity was discovered and the L-fucose pathway converts L-galactose to dihydroxyacetone phosphate and L-glyceraldehyde, which is toxic.
...
...

References

SHOWING 1-10 OF 24 REFERENCES
Studies on D-ribulose and its enzymatic conversion to D-arabinose.
  • S. Cohen
  • Biology
    The Journal of biological chemistry
  • 1953
METABOLISM OF PENTOSES AND PENTITOLS BY AEROBACTER AEROGENES I
TLDR
The versatility of this strain of A. aerogenes is due to an ability to synthesize in the presence of appropriate carbohydrates (inducers) families of enzymes which catalyze the metabolism of the carbohydrates (i.e., families of pentitol dehydrogenases, aldopentose isomerases, and pentulokinases).
D-phosphoarabinoisomerase and D-ribulokinase in Escherichia coli.
METABOLISM OF CARBOHYDRATES BY PSEUDOMONAS SACCHAROPHILA III
TLDR
Four different pathways for the oxidation of various hexose and pentose sugars have been found in Pseudomonas saccharophila, and the initial steps involve a DPN-linked oxidation of the sugars to the corresponding acids and the subsequent enzymatic hydrolysis of the lactones.
Purification and properties of lactaldehyde dehydrogenase from Escherichia coli.
Regulation of glyoxylate metabolism in Escherichia coli K-12.
TLDR
Evidence indicates that malate synthase G plays an anaplerotic role during growth with glycolate or acetate as the carbon source, and that wild-type organisms convert glyoxylate to acetyl-coenzyme A and oxidize it via the tricarboxylic acid cycle.
A new spectrophotometric method for the detection and determination of keto sugars and trioses.
GENETIC ASPECTS OF METABOLIC CONTROL.
TLDR
The induction process is a two-step process that leads to repression and the goal is to eliminate the source of repression.
...
...