Metabolism of cellulose by Phanerochaete chrysosporium in continuously agitated culture is associated with enhanced production of lignin peroxidase.

@article{Zacchi2000MetabolismOC,
  title={Metabolism of cellulose by Phanerochaete chrysosporium in continuously agitated culture is associated with enhanced production of lignin peroxidase.},
  author={Laura Zacchi and G Burla and D Zuolong and Patricia J. Harvey},
  journal={Journal of biotechnology},
  year={2000},
  volume={78 2},
  pages={185-92}
}
Production of the extracellular heme protein lignin peroxidase (LiP) by Phanerochaete chrysosporium is currently associated with a number of requirements, namely exposure of the cultures to oxygen; limiting nutrient nitrogen or carbon and static or semi-static culture conditions. To obtain LiP activity in continuously agitated liquid culture requires the inclusion of a surfactant. However, using cellulose as the carbon source, we obtained high titres (0.2-0.4 U ml(-1)) of LiP in submerged… CONTINUE READING

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Comparison of the morphological and physiological traits of hyphae maintained on either cellulose or free glucose supports observations that the synthesis of extracellular polysaccharide in the cultures grown on glucose , restricts oxygen diffusion into the hyphae , which is necessary for LiP induction .
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