Metabolism of all-trans-retinoic acid in hamster liver microsomes: oxidation of 4-hydroxy- to 4-keto-retinoic acid.

  title={Metabolism of all-trans-retinoic acid in hamster liver microsomes: oxidation of 4-hydroxy- to 4-keto-retinoic acid.},
  author={Anjeanette Roberts and Lois C. Lamb and Michael B. Sporn},
  journal={Archives of biochemistry and biophysics},
  volume={199 2},

Biliary metabolites of all-trans-retinoic acid in the rat.

Retinoic acid metabolism by a system reconstituted with cytochrome P-450.

Tissue dependence of retinoic acid metabolism in vivo.


The results indicated that the 4-hydroxylation of RA, catalyzed prenatally by CYP3A7, might play an important role in protecting the human fetus against RA-induced embryotoxins and teratogens.

Biliary metabolites of all-trans-retinoic acid in the rat: isolation and identification of a novel polar metabolite.

This metabolite represents the first taurine conjugate of a fat-soluble vitamin to be identified and accounts for 10% of the total biliary radioactivity.

Regulation of CYP26 (cytochrome P450RAI) mRNA expression and retinoic acid metabolism by retinoids and dietary vitamin A in liver of mice and rats

CYP26 expression and RA metabolism are regulated in adult liver not only acutely by RA administration, as may be relevant to retinoid therapy, but under chronic dietary conditions relevant to vitamin A nutrition in humans.

Highly specific cytochrome P450-like enzymes for all-trans-retinoic acid in T47D human breast cancer cells.

  • I. HanJ. H. Choi
  • Biology, Chemistry
    The Journal of clinical endocrinology and metabolism
  • 1996
The RA-inducible 4- and 18-hydroxylases showed high specificity for RA and high levels of catalytic activities, with Km and maximum velocity values for 4-Hydroxylase equal to 99 nmol/L and 0.26 pmol/ protein, respectively.



In vitro metabolism and biological activity of all-trans-retinoic acid and its metabolites in hamster trachea.

The in vitro metabolism of all-trans-[11,12-3h]retinoic acid to several more polar compounds has been demonstrated in a hamster tracheal organ culture system and one of the metabolites shows about one-tenth the biological activity of all.

Pathways of retinol and retinoic acid metabolism in the rat.

Evidence is presented that retinoic acid and retinol are metabolized by either the same or at least similar pathways and thatretinol becomes oxidized to the carboxyl state before any degradation of the isoprenoid side chain occurs.

Isolation and identification of three major metabolites of retinoic acid from rat feces.

Following the intraperitoneal administration of high doses of 14C- and 3H- labelled retinoic acid (1) to rats, three major metabolites and the intact compound were isolated from the feces in

Isolation and identification of 4-hydroxy- and 4-oxoretinoic acid. In vitro metabolites of all-trans-retinoic acid in hamster trachea and liver.

Two metabolites produced in a cell-free liver incubation reaction also migrate on a high-pressure liquid chromatography column together with metabolites isolated from a tracheal organ culture system both in ultraviolet absorption and mass spectral characteristics and in migration rates on two different reverse-phase high- pressure liquid chromatographic systems.

Biosynthesis of beta-glucuronides of retinol and of retinoic acid in vivo and in vitro.

The conversion of retinal to retinoic acid and the synthesis of retinoyl -glucuronide from retinoIC acid could not be detected in whole homogenates, cell fractions, or outer segments of the bovine retina.

Isolation and identification of three urinary metabolites of retinoic acid in the rat.

After the intraperitoneal administration of high doses of 14C- and 3H-labelled retinoic acid (1) to rats three major urinary metabolites have been isolated in microgram amounts by use of column,

Isolation and identification of 5, 6-epoxyretinoic acid: a biologically active metabolite of retinoic acid.

5, 8-oxyretinoic acid previously isolated in this laboratory from intestinal mucosa was probably generated from 5, 6-epoxyretinosic acid by the acidic conditions used in the extraction and isolation of the 5, 8, 5, 7, 6, and 8III.