Metabolism of 3-methylindole by vaccinia-expressed P450 enzymes: correlation of 3-methyleneindolenine formation and protein-binding.

@article{ThorntonManning1996MetabolismO3,
  title={Metabolism of 3-methylindole by vaccinia-expressed P450 enzymes: correlation of 3-methyleneindolenine formation and protein-binding.},
  author={Janice R. Thornton-Manning and Michael Appleton and Frank J. Gonzalez and Garold S. Yost},
  journal={The Journal of pharmacology and experimental therapeutics},
  year={1996},
  volume={276 1},
  pages={21-9}
}
The toxicity of 3-methylindole (3 MI), a selective pneumotoxin, is dependent upon cytochrome P450-mediated bioactivation 3. Using vaccinia-expressed P450 enzymes, the metabolites of radiolabeled 3 MI produced by 14 individual P450s were identified and quantified by high performance liquid chromatography. Indole-3-carbinol was produced from incubations of 3 MI with only four enzymes. Although 3-methyloxindole was a product of several P450s, human 1A2 was most efficient in producing this… CONTINUE READING

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