Metabolism of 1alpha,25(OH)2D3 and its 20-epi analog integrates clonal expansion, maturation and apoptosis during HL-60 cell differentiation.

@article{Campbell1999MetabolismO1,
  title={Metabolism of 1alpha,25(OH)2D3 and its 20-epi analog integrates clonal expansion, maturation and apoptosis during HL-60 cell differentiation.},
  author={Moray J. Campbell and Mark Trehane Drayson and Jennifer Durham and L A Wallington and Mei-Lung Siu-Caldera and Gudimetla Satyanarayana Reddy and Geoffrey Brown},
  journal={Molecular and cellular endocrinology},
  year={1999},
  volume={149 1-2},
  pages={169-83}
}
Induction of growth arrest and monocyte differentiation of HL-60 leukemia cells by 1alpha,25 dihydroxyvitamin D3 (1alpha,25(OH)2D3) is well established. By contrast, we have observed, that 1alpha,25(OH)2D3 and its metabolites play separate roles in clonal expansion and survival of differentiating HL-60 cells. Cells that had differentiated by 48 h (CD14 positive) grew slower than control cells, whereas CD14 negative cells were growing faster at this time point. Inhibiting 1alpha,25(OH)2D3 or… CONTINUE READING