Metabolic studies in isolated rat liver cells: I. Lipid synthesis

  title={Metabolic studies in isolated rat liver cells: I. Lipid synthesis},
  author={David M. Capuzzi and S. Margolis},
Rat liver cells, isolated by chelate perfusion and extrusion through a tissue press, incorporated labeled acetate into cellular lipids and into lipids released into the suspending medium. Optimal rates of incorporation required supplementation of tris-KCl medium with Mg++, Mn++, succinate, citrate, nicotinamide, Coenzyme A, NADP and glucose-6-phosphate. The rate of acetate incorporation was markedly altered by changes in incubation media; tris-KCl was the most effective buffer. All the major… CONTINUE READING

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Manery, Can

  • G. J. Marcus, J.G
  • J. Biochem
  • 1966
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