Metabolic profiling of Escherichia coli cultivations: evaluation of extraction and metabolite analysis procedures

@article{Hiller2007MetabolicPO,
  title={Metabolic profiling of Escherichia coli cultivations: evaluation of extraction and metabolite analysis procedures},
  author={Julia Hiller and Ezequiel Franco-Lara and Dirk Weuster-Botz},
  journal={Biotechnology Letters},
  year={2007},
  volume={29},
  pages={1169-1178}
}
The quantitative estimation of intracellular metabolite concentrations (metabolic profiling) is a prerequisite for a better understanding of biological processes and thus inevitable for the rational improvement of microbial production strains and process design. Since pool sizes of substrates regulate flux through different enzymes, the accurate determination of intracellular metabolite concentrations is necessary to understand in vivo reaction kinetics. Quantification of intracellular… CONTINUE READING
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