Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts

  title={Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts},
  author={O. Beckonert and H. Keun and T. Ebbels and J. Bundy and E. Holmes and J. Lindon and J. Nicholson},
  journal={Nature Protocols},
  • O. Beckonert, H. Keun, +4 authors J. Nicholson
  • Published 2007
  • Biology, Medicine
  • Nature Protocols
  • Metabolic profiling, metabolomic and metabonomic studies mainly involve the multicomponent analysis of biological fluids, tissue and cell extracts using NMR spectroscopy and/or mass spectrometry (MS). We summarize the main NMR spectroscopic applications in modern metabolic research, and provide detailed protocols for biofluid (urine, serum/plasma) and tissue sample collection and preparation, including the extraction of polar and lipophilic metabolites from tissues. 1H NMR spectroscopic… CONTINUE READING
    1,412 Citations

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