Metabolic pathway of 6-aminohexanoate in the nylon oligomer-degrading bacterium Arthrobacter sp. KI72: identification of the enzymes responsible for the conversion of 6-aminohexanoate to adipate

  title={Metabolic pathway of 6-aminohexanoate in the nylon oligomer-degrading bacterium Arthrobacter sp. KI72: identification of the enzymes responsible for the conversion of 6-aminohexanoate to adipate},
  author={Ikki Takehara and Tsubasa Fujii and Yuuki Tanimoto and Dai-ichiro Kato and Masahiro Takeo and Seiji Negoro},
  journal={Applied Microbiology and Biotechnology},
Arthrobacter sp. strain KI72 grows on a 6-aminohexanoate oligomer, which is a by-product of nylon-6 manufacturing, as a sole source of carbon and nitrogen. We cloned the two genes, nylD1 and nylE1, responsible for 6-aminohexanoate metabolism on the basis of the draft genomic DNA sequence of strain KI72. We amplified the DNA fragments that encode these genes by polymerase chain reaction using a synthetic primer DNA homologous to the 4-aminobutyrate metabolic enzymes. We inserted the amplified… 
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Identification of a Transcriptional Activator (ChnR) and a 6-Oxohexanoate Dehydrogenase (ChnE) in the Cyclohexanol Catabolic Pathway in Acinetobacter sp. Strain NCIMB 9871 and Localization of the Genes That Encode Them
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Biodegradation of nylon oligomers
  • S. Negoro
  • Biology, Engineering
    Applied Microbiology and Biotechnology
  • 2000
A molecular basis for the adaptation of microorganisms toward xenobiotics compounds was described and two strains which initially had no metabolic activity for nylon oligomers, Flavobacterium sp.
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Metabolic Engineering toward Sustainable Production of Nylon-6.
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Nylon-oligomer degrading enzyme/substrate complex: catalytic mechanism of 6-aminohexanoate-dimer hydrolase.
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