Metabolic network analysis and experimental study of lipid production in Rhodosporidium toruloides grown on single and mixed substrates

@article{Bommareddy2015MetabolicNA,
  title={Metabolic network analysis and experimental study of lipid production in Rhodosporidium toruloides grown on single and mixed substrates},
  author={Rajesh Reddy Bommareddy and Wael Sabra and Garima Maheshwari and An-Ping Zeng},
  journal={Microbial Cell Factories},
  year={2015},
  volume={14}
}
BackgroundMicrobial lipids (triacylglycerols, TAG) have received large attention for a sustainable production of oleochemicals and biofuels. Rhodosporidium toruloides can accumulate lipids up to 70% of its cell mass under certain conditions. However, our understanding of lipid production in this yeast is still much limited, especially for growth with mixed substrates at the level of metabolic network. In this work, the potentials of several important carbon sources for TAG production in R… 
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Highly Influential Citations
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104 Citations

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Comprehensive analysis of a metabolic model for lipid production in Rhodosporidium toruloides.
Proteome analysis of xylose metabolism in Rhodotorula toruloides during lipid production
TLDR
It is suggested that sugar import is the limiting step during xylose conversion by R. toruloides into lipids, and increases in enzyme levels of both fatty acid biosynthesis and beta-oxidation inxylose-grown cells was predicted to result in a futile cycle.
Proteome analysis of xylose metabolism in Rhodotorula toruloides during lipid production
TLDR
It is suggested that sugar import is the limiting step during xylose conversion by R. toruloides into lipids, and NADPH appeared to be regenerated primarily through pentose phosphate pathway although it may also involve malic enzyme as well as alcohol and aldehyde dehydrogenases.
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A metabolic model of Lipomyces starkeyi for predicting lipogenesis potential from diverse low-cost substrates
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The lipid biosynthesis potential of L. starkeyi can be significantly improved through appropriate modification of metabolic network, as well as combined utilization of carbon sources according to the metabolic model, which provides valuable guidance to improve lipid production from various low-cost substrates.
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The simulation results confirmed that the R. toruloides model provides valid growth predictions on glucose, xylose, and glycerol, while prediction of genetic engineering targets to increase production of linolenic acid, triacylglycerols, and carotenoids identified genes—some of which have previously been engineered to successfully increase production.
Engineering Rhodosporidium toruloides for increased lipid production
TLDR
This work represents one of the first examples of metabolic engineering in R. toruloides and establishes this yeast as a new platform for production of fatty‐acid derived products.
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TLDR
Transcriptional profiles showed that the regulation of glycerol uptake in the presence of glucose at transcriptional level is different from that observed in Saccharomyces cerevisiae, and insights into lipid biosynthesis on mixed substrates are provided.
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TLDR
A general approach for barcoded mutagenesis that should enable functional genomics in diverse fungi is demonstrated and a high-confidence set of 150 genes affecting lipid accumulation is identified, including genes with predicted function in signaling cascades, gene expression, protein modification and vesicular trafficking, autophagy, amino acid synthesis and tRNA modification.
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