Metabolic labelling of oligosaccharide chains of glycoproteins in a rat myoblast cell line and its lectin resistant mutants.

Abstract

We have determined the experimental conditions under which optimum hydrolysis of the various oligosaccharides of membrane glycopeptides can be achieved in a permanent line of rat skeletal myoblasts and its wheat germ agglutinin resistant mutants. The labelled glycopeptides were isolated by extensive pronase digestion of whole cells. Labelling studies demonstrated that label from fucose and galactose remained largely in these two sugars, while glucosamine labelled sialic acid and N-acetylglucosamine. Mannose label was found in both mannose and fucose.

Cite this paper

@article{Gilfix1983MetabolicLO, title={Metabolic labelling of oligosaccharide chains of glycoproteins in a rat myoblast cell line and its lectin resistant mutants.}, author={Brian M. Gilfix and B. D. Sanwal}, journal={Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire}, year={1983}, volume={61 10}, pages={1129-32} }