Metabolic functions of biotin. IV. The rôle of carbamyl-l-glutamic acid in the synthesis of citrulline by normal and biotin-deficient rats.

@article{FELDOTT1951MetabolicFO,
  title={Metabolic functions of biotin. IV. The r{\^o}le of carbamyl-l-glutamic acid in the synthesis of citrulline by normal and biotin-deficient rats.},
  author={G FELDOTT and Henry A. Lardy},
  journal={The Journal of biological chemistry},
  year={1951},
  volume={192 1},
  pages={447-52}
}
A characteristic consequence of a biotin deficiency in animals is a decreased ability to fix C402 into various tissue components including the amino acid arginine (1). Carbon dioxide is incorporated into arginine as a result of the conversion of ornithine to citrulline in the Krebs-Henseleit urea cycle (2). The methods developed by Cohen and Hayano (3, 4) for the synthesis of citrulline from ornithine, glutamate, COz, and ammonia by washed residue of rat liver homogenate have been applied to… CONTINUE READING