Metabolic exploitation of the sialic acid biosynthetic pathway to generate site-specifically labeled antibodies.

@article{Rochefort2014MetabolicEO,
  title={Metabolic exploitation of the sialic acid biosynthetic pathway to generate site-specifically labeled antibodies.},
  author={Matthew M. Rochefort and Mark D. Girgis and Jacob S. Ankeny and James S Tomlinson},
  journal={Glycobiology},
  year={2014},
  volume={24 1},
  pages={62-9}
}
Lack of a universal site-specific conjugation methodology for antibodies limits their potential to be developed as tumor-specific imaging agents or targeted therapeutics. A potential mechanism for site-specific conjugation involves utilization of the conserved N-glycosylation site in the CH2 domain. We sought to develop an antibody with an altered azido-sugar at this site whereby site-specific label could be added. The HB8059 hybridoma was cultured with peracetylated N-azidoacetlymannosamine… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 26 references

Covalent disul fi delinked anti - CEA diabody allows site - speci fi c conjugation and radi - olabeling for tumor targeting applications

  • T Olafsen, CW Cheung, +7 authors AA Raubitschek
  • Protein Eng Des Sel
  • 2004
1 Excerpt

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