Metabolic engineering.

@article{Stephanopoulos1998MetabolicE,
  title={Metabolic engineering.},
  author={G. Stephanopoulos},
  journal={Biotechnology and bioengineering},
  year={1998},
  volume={58 2-3},
  pages={
          119-20
        }
}
Metabolic engineering is the science that combines systematic analysis of metabolic and other pathways with molecular biological techniques to improve cellular properties by designing and implementing rational genetic modifications. As such, metabolic engineering deals with the measurement of metabolic fluxes and elucidation of their control as determinants of metabolic function and cell physiology. A novel aspect of metabolic engineering is that it departs from the traditional reductionist… Expand
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References

SHOWING 1-10 OF 98 REFERENCES
Metabolic fluxes and metabolic engineering.
TLDR
It is shown how metabolic fluxes can be used in the systematic elucidation of metabolic control in the framework of reaction grouping and top-down metabolic control analysis. Expand
Toward a science of metabolic engineering
Application of recombinant DNA methods to restructure metabolic networks can improve production of metabolite and protein products by altering pathway distributions and rates. Recruitment ofExpand
Network rigidity and metabolic engineering in metabolite overproduction
TLDR
This work has shown that overproduction of many metabolites requires significant redirection of flux distributions in the primary metabolism, which may not readily occur following product deregulation because metabolic pathways have evolved to exhibit control architectures that resist flux alterations at branch points. Expand
Flux amplification in complex metabolic networks
A new methodology is presented for the analysis of complex metabolic networks with the goal of overproduction of metabolites. The objective is to locate a small number of reaction steps in a networkExpand
New directions in metabolic engineering.
TLDR
The genetic modification of cellular biochemistry to introduce new properties or modify existing ones and new problems and technologies are constantly being developed. Expand
Combinatorial biosynthesis for new drug discovery.
  • C. Hutchinson
  • Biology, Medicine
  • Current opinion in microbiology
  • 1998
TLDR
Extensions of the approach to include deoxysugar biosynthesis genes have expanded the possibilities for making new microbial metabolites and discovering valuable drugs through the genetic engineering of bacteria. Expand
Metabolic Engineering of Propanediol Pathways
Microbial fermentation is an important technology for the conversion of renewable resources to chemicals. In this paper, we describe the application of metabolic engineering for the development ofExpand
Increased Carotenoid Production by the Food YeastCandida utilis through Metabolic Engineering of the Isoprenoid Pathway
TLDR
Through metabolic engineering of the isoprenoid pathway, a sevenfold increase in the yield of lycopene has been achieved through modifications in related biochemical pathways can be utilized to enhance the production of commercially desirable compounds such as carotenoids. Expand
Metabolic fluxes in riboflavin-producing Bacillus subtilis
TLDR
The pentose phosphate pathway and the pyruvate shunt were identified as major pathways of glucose catabolism in a recombinant, riboflavin-producing Bacillus subtilis strain, and the overall flux distribution suggests that B. subtilIS metabolism has an unusually high capacity for the reoxidation of NADPH. Expand
Metabolic flux determination in C6 glioma cells using carbon-13 distribution upon [1-13C]glucose incubation.
TLDR
The model was used for calculating metabolic fluxes in a rat tumor cell line, the C6 glioma, incubated with [1-13C]glucose, and the results emphasize different metabolic characteristics of C6 cells when compared to astrocytes, their normal counterpart. Expand
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