Metabolic engineering.

  title={Metabolic engineering.},
  author={Gregory Stephanopoulos},
  journal={Biotechnology and bioengineering},
  volume={58 2-3},
Metabolic engineering is the science that combines systematic analysis of metabolic and other pathways with molecular biological techniques to improve cellular properties by designing and implementing rational genetic modifications. As such, metabolic engineering deals with the measurement of metabolic fluxes and elucidation of their control as determinants of metabolic function and cell physiology. A novel aspect of metabolic engineering is that it departs from the traditional reductionist… 
Metabolic engineering of Escherichia coli for the efficient utilization of plant sugar mixture
The flux analysis revealed that slow growth of ptsG mutant is due to less efficient growth, which results in low yields and productivities of the desired product.
Nonstationary metabolic flux analysis (NMFA) for the elucidation of cellular physiology
A software package (MetranCL) is built that combines the elementary metabolite unit (EMU) framework, a new network decomposition strategy termed block decoupling, and a customized differential equation solver that performs flux estimations as much as 5000 times faster than the previous state-of-the-art NMFA methods.
How will bioinformatics influence metabolic engineering?
This article shows how the genomically defined microbial metabolic genotypes can be analyzed by FBA, and discusses the advantage of this approach, and how FBA is expected to find uses in the near future.
Modelling strategies for the industrial exploitation of lactic acid bacteria
The industrial applications of LAB are mapped onto available global, genome-scale metabolic modelling techniques to evaluate the extent to which functional genomics and systems biology can live up to their industrial promise.
Quantitative analysis of relationships between fluxome and metabolome in Escherichia coli
A growth rate dependent biomass composition was derived and the resulting metabolic network model only requires the specific rate of growth, μ, as an input in order to accurately predict all other fluxes and yields.
Modular model-based design for heterologous bioproduction in bacteria.
A study on fatty acid ethyl ester production in a Saccharomyces cerevisiae cell factory
Three different metabolic engineering strategies were applied to improve the production of FAEEs in Saccharomyces cerevisiae, pointing out the increased cellular stress that influenced cellular growth and the increased demand of cofactor NADPH which is therefore considered an engineering target for the future.
Gluconeogenesis as a system : development of in vivo flux analysis of hepatic glucose production in Type 2 Diabetes
ized and Polarized Epithelial Cells: a CFTRand Caveolin-1-Dependent Process  Computational modeling of loCal intravasCular drug delivery  An IntegrAted ComputAtIonAl ApproACh for the determInAtIon
Strategies and tools to improve crop productivity by targeting photosynthesis
This work is interested in identifying control points in maize photoassimilation that are amenable to gene manipulation to improve overall productivity and provide step change advancement in overall crop productivity.


Metabolic fluxes and metabolic engineering.
It is shown how metabolic fluxes can be used in the systematic elucidation of metabolic control in the framework of reaction grouping and top-down metabolic control analysis.
Toward a science of metabolic engineering
Application of recombinant DNA methods to restructure metabolic networks can improve production of metabolite and protein products by altering pathway distributions and rates. Recruitment of
Network rigidity and metabolic engineering in metabolite overproduction
This work has shown that overproduction of many metabolites requires significant redirection of flux distributions in the primary metabolism, which may not readily occur following product deregulation because metabolic pathways have evolved to exhibit control architectures that resist flux alterations at branch points.
Flux amplification in complex metabolic networks
New directions in metabolic engineering.
Combinatorial biosynthesis for new drug discovery.
  • C. Hutchinson
  • Biology, Chemistry
    Current opinion in microbiology
  • 1998
Increased Carotenoid Production by the Food YeastCandida utilis through Metabolic Engineering of the Isoprenoid Pathway
Through metabolic engineering of the isoprenoid pathway, a sevenfold increase in the yield of lycopene has been achieved through modifications in related biochemical pathways can be utilized to enhance the production of commercially desirable compounds such as carotenoids.
Metabolic fluxes in riboflavin-producing Bacillus subtilis
The pentose phosphate pathway and the pyruvate shunt were identified as major pathways of glucose catabolism in a recombinant, riboflavin-producing Bacillus subtilis strain, and the overall flux distribution suggests that B. subtilIS metabolism has an unusually high capacity for the reoxidation of NADPH.
Metabolic flux determination in C6 glioma cells using carbon-13 distribution upon [1-13C]glucose incubation.
The model was used for calculating metabolic fluxes in a rat tumor cell line, the C6 glioma, incubated with [1-13C]glucose, and the results emphasize different metabolic characteristics of C6 cells when compared to astrocytes, their normal counterpart.
Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells
Effective cell-cycle arrest of Chinese hamster ovary cells is achieved by tetracy-cline-regulated coexpression of p21 and the differentiation factor CCAAT/enhancer-binding protein α (which both stabilizes and induces p21), and production of a model heterologous protein (secreted alkaline phosphatase; SEAP) has been increased.