Metabolic engineering.

@article{Stephanopoulos1998MetabolicE,
  title={Metabolic engineering.},
  author={G. Stephanopoulos},
  journal={Biotechnology and bioengineering},
  year={1998},
  volume={58 2-3},
  pages={
          119-20
        }
}
Metabolic engineering is the science that combines systematic analysis of metabolic and other pathways with molecular biological techniques to improve cellular properties by designing and implementing rational genetic modifications. As such, metabolic engineering deals with the measurement of metabolic fluxes and elucidation of their control as determinants of metabolic function and cell physiology. A novel aspect of metabolic engineering is that it departs from the traditional reductionist… Expand
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31 Citations
Highly Influential Citations
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31 Citations

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Metabolic engineering of Escherichia coli for the efficient utilization of plant sugar mixture
In recent years, metabolic flux analysis (MFA) has become an important tool in metabolic engineering. The MFA involves the quantification of intracellular metabolic fluxes in a microorganism. The… Expand
Nonstationary metabolic flux analysis (NMFA) for the elucidation of cellular physiology
Many current and future applications of biological engineering hinge on our ability to measure, understand, and manipulate metabolism. Many diseases for which we seek cures are metabolic in nature.… Expand
Progress in Metabolic Engineering of Saccharomyces cerevisiae
  • E. Nevoigt
  • Medicine, Biology
  • Microbiology and Molecular Biology Reviews
  • 2008
TLDR
The current review discusses the relevance of several engineering strategies, such as rational and inverse metabolic engineering, evolutionary engineering, and global transcription machinery engineering, in yeast strain improvement and summarizes existing tools for fine-tuning and regulating enzyme activities and thus metabolic pathways. Expand
How will bioinformatics influence metabolic engineering?
TLDR
This article shows how the genomically defined microbial metabolic genotypes can be analyzed by FBA, and discusses the advantage of this approach, and how FBA is expected to find uses in the near future. Expand
Modelling strategies for the industrial exploitation of lactic acid bacteria
TLDR
The industrial applications of LAB are mapped onto available global, genome-scale metabolic modelling techniques to evaluate the extent to which functional genomics and systems biology can live up to their industrial promise. Expand
Quantitative analysis of relationships between fluxome and metabolome in Escherichia coli
Metabolic network models describing growth of Escherichia coli on glucose, glycerol and acetate were derived from a genome-scale model of E. coli. One of the uncertainties in the metabolic networks… Expand
Functional similarities of reaction sets in metabolic pathways
TLDR
A novel method is developed that first transforms the polyhedral cones to polytopes and then uses minimum enclosing balls to approximate this intersection efficiently and is of great use for both measuring the impacts of pathway components and identifying functionally similar component sets. Expand
Modular model-based design for heterologous bioproduction in bacteria.
TLDR
It is shown how recent characterization of genetic parts could be used to fine-tune the expression of individual genes to achieve that goal, and fastens the design of metabolic circuits using a combinatorial design approach. Expand
Systems biotechnology of animal cells: the road to prediction.
TLDR
An overview of the main trends towards the production of sufficient quantities of recombinant products from manufacturing processes based on animal cell culture is provided, with a focus on metabolic models as central scaffolds for data integration and prediction of bioprocess outcomes. Expand
A study on fatty acid ethyl ester production in a Saccharomyces cerevisiae cell factory
Currently there is an obvious demand for alternative transportation fuels to fulfill the requirements for the future transportation. Fatty acid ethyl esters (FAEEs) have chemical properties that are… Expand
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References

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Microbial fermentation is an important technology for the conversion of renewable resources to chemicals. In this paper, we describe the application of metabolic engineering for the development of… Expand
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TLDR
Through metabolic engineering of the isoprenoid pathway, a sevenfold increase in the yield of lycopene has been achieved through modifications in related biochemical pathways can be utilized to enhance the production of commercially desirable compounds such as carotenoids. Expand
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