Metabolic engineering to enhance bacterial hydrogen production

  title={Metabolic engineering to enhance bacterial hydrogen production},
  author={Toshinari Maeda and Viviana Sanchez‐Torres and Thomas K. Wood},
  booktitle={Microbial biotechnology},
Hydrogen fuel is renewable, efficient and clean, and fermentative bacteria hold great promise for its generation. Here we use the isogenic Escherichia coli K-12 KEIO library to rapidly construct multiple, precise deletions in the E. coli genome to direct the metabolic flux towards hydrogen production. Escherichia coli has three active hydrogenases, and the genes involved in the regulation of the formate hydrogen lyase (FHL) system for synthesizing hydrogen from formate via hydrogenase 3 were… CONTINUE READING