Metabolic engineering strategies for enhanced shikimate biosynthesis: current scenario and future developments

  title={Metabolic engineering strategies for enhanced shikimate biosynthesis: current scenario and future developments},
  author={M. Bilal and Songwei Wang and H. Iqbal and Yuping Zhao and Hong-bo Hu and W. Wang and Xuehong Zhang},
  journal={Applied Microbiology and Biotechnology},
Shikimic acid is an important intermediate for the manufacture of the antiviral drug oseltamivir (Tamiflu®) and many other pharmaceutical compounds. Much of its existing supply is obtained from the seeds of Chinese star anise (Illicium verum). Nevertheless, plants cannot supply a stable source of affordable shikimate along with laborious and cost-expensive extraction and purification process. Microbial biosynthesis of shikimate through metabolic engineering and synthetic biology approaches… Expand
Metabolic engineering of Escherichia coli for production of chemicals derived from the shikimate pathway
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Extending the shikimate pathway for microbial production of maleate from glycerol in engineered Escherichia coli
This study provided a promising microbial platform for industrial level synthesis ofmaleate, and demonstrated the highest titer of maleate production in microorganisms so far. Expand
Rewiring central carbon metabolism for tyrosol and salidroside production in Saccharomyces cerevisiae
Tyrosol production from glucose by S. cerevisiae was engineered with a push‐pull‐restrain strategy and the total amount of tyrosol and salidroside with glucose fed‐batch fermentation was over 10 g/L and reached levels suitable for large‐scale production. Expand
Cell Factory Design and Culture Process Optimization for Dehydroshikimate Biosynthesis in Escherichia coli
An engineered Escherichia coli cell factory is created to produce a high titer of DHS as well as an efficient system for the conversion DHS into MA, suggesting that the rational cell factory design of DHS and MA biosynthesis could be a feasible way to complement petrochemical-based chemical processes. Expand
Calophyllum brasiliense Cambess: An alternative and promising source of shikimic acid
  • Rogério Marchiosi, Ana Paula Ferro, +5 authors Osvaldo Ferrarese-Filho
  • Chemistry
  • 2019
Abstract Shikimic acid is the starting material for the synthesis of the antiviral oseltamivir phosphate, a front-line combatant against the human influenza A and B viruses. Nowadays, the demand forExpand
Plant Secondary Metabolites with an Overview of Populus
Compared with bacteria, fewer known pathways produce secondary metabolites in Populus despite P. trichocarpa having had its genome sequenced, leading to more studies of secondary metabolites and their biosynthesis. Expand
Common problems associated with the microbial productions of aromatic compounds and corresponding metabolic engineering strategies.
The common problems associated with the microbial biosynthesis of diverse aromatic compounds are described and the corresponding metabolic engineering strategies for resolving these problems are summarized. Expand
New insights into transport capability of sugars and its impact on growth from novel mutants of Escherichia coli
The major sugar transport systems for glucose and disaccharide transport, the exhibited substrate plasticity, and their impact on the growth of E. coli are summarized, highlighting the relevance of PTS in the control of the expression of genes for the transport and catabolism of other sugars as xylose. Expand
Aromatic Compound Production by Corynebacterium glutamicum
Aromatic compounds represent important bulk and fine chemicals with numerous applications in the food, feed, cosmetics, pharmaceutical, and chemical industries. These are currently produced fromExpand
Electricigens in the anode of microbial fuel cells: pure cultures versus mixed communities
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Novel technologies combined with traditional metabolic engineering strategies facilitate the construction of shikimate-producing Escherichia coli
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Innovating a Nonconventional Yeast Platform for Producing Shikimate as the Building Block of High-Value Aromatics.
The development of a new platform based on Scheffersomyces stipitis, whose superior xylose utilization efficiency makes it particularly suited to produce the shikimate group of compounds, which was produced at 3.11 g/L, representing the highest level amongShikimate pathway products in yeasts. Expand
Metabolic engineering for microbial production of shikimic acid.
Engineering the uptake of carbon, the regulatory circuits, central metabolism and the common aromatic pathway including shikimic acid import that have all been targeted to effect higher productivities and lower by-product formation are discussed. Expand
Metabolic engineering of Escherichia coli for improving shikimate synthesis from glucose.
An overproducing shikimate Escherichia coli strain was developed by rationally engineering certain metabolic pathways to achieve this, and three critical enzymes of mutated 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase, PEP synthase and transketolase A were modularly overexpressed. Expand
Shikimic Acid Production in Escherichia coli: From Classical Metabolic Engineering Strategies to Omics Applied to Improve Its Production
The main metabolic engineering strategies that have been applied for the development of efficient SA-producing strains are discussed, as the perspective of omics analysis has focused on further strain improvement for the production of this valuable aromatic intermediate. Expand
Production of shikimic acid from Escherichia coli through chemically inducible chromosomal evolution and cofactor metabolic engineering
It is presented the first demonstration that increasing NADPH availability by overexpressing the pntAB or nadK genes significantly enhances SA production, and an E. coli strain that carries neither a plasmid nor an antibiotic marker was constructed by triclosan-induced chromosomal evolution. Expand
Engineering the shikimate pathway for biosynthesis of molecules with pharmaceutical activities in E. coli.
  • M. Jiang, Haoran Zhang
  • Biology, Medicine
  • Current opinion in biotechnology
  • 2016
This review summarizes successful and generally applicable strategies for engineering this important pathway in the context of the model bacterium E. coli for biosynthesis of molecules with pharmaceutical activities. Expand
Tunable switch mediated shikimate biosynthesis in an engineered non-auxotrophic Escherichia coli
The tunable switch developed in this study is an efficient tool for regulating indispensable genes involved in critical metabolic pathways that can conditionally decrease gene expression and substituted it for the original aroK promoters. Expand
Engineering Pseudomonas for phenazine biosynthesis, regulation, and biotechnological applications: a review
The biosynthetic pathway and regulatory mechanism involved in the phenazine biosynthesis are comprehensively discussed, and a summary of biological functionalities and biotechnological applications of the phenazines is provided. Expand
Expanding horizons of shikimic acid
The present review attempts to focus on the market trend of shikimic acid due to its high demand with particular emphasis laid on the pandemics of swine flu. Expand