Metabolic engineering strategies for enhanced shikimate biosynthesis: current scenario and future developments

  title={Metabolic engineering strategies for enhanced shikimate biosynthesis: current scenario and future developments},
  author={Muhammad Bilal and Songwei Wang and Hafiz M.N. Iqbal and Yuping Zhao and Hong-bo Hu and W. Wang and Xuehong Zhang},
  journal={Applied Microbiology and Biotechnology},
Shikimic acid is an important intermediate for the manufacture of the antiviral drug oseltamivir (Tamiflu®) and many other pharmaceutical compounds. Much of its existing supply is obtained from the seeds of Chinese star anise (Illicium verum). Nevertheless, plants cannot supply a stable source of affordable shikimate along with laborious and cost-expensive extraction and purification process. Microbial biosynthesis of shikimate through metabolic engineering and synthetic biology approaches… 

Extending the shikimate pathway for microbial production of maleate from glycerol in engineered Escherichia coli

This study provided a promising microbial platform for industrial level synthesis ofmaleate, and demonstrated the highest titer of maleate production in microorganisms so far.

Rewiring central carbon metabolism for tyrosol and salidroside production in Saccharomyces cerevisiae

Tyrosol production from glucose by S. cerevisiae was engineered with a push‐pull‐restrain strategy and the total amount of tyrosol and salidroside with glucose fed‐batch fermentation was over 10 g/L and reached levels suitable for large‐scale production.

Cofactor Self-Sufficient Whole-Cell Biocatalysts for the Relay-Race Synthesis of Shikimic Acid

The efficient relay-race synthesis of SA from glucose by coupling microbial fermentation with a biocatalytic process was finally achieved and provides an effective strategy for the biosynthesis of fine chemicals that are difficult to obtain through de novo biosynthesis from renewable feedstocks, as well as for bioc atalytic studies that strictly rely on NAD(P)H regeneration.

Cell Factory Design and Culture Process Optimization for Dehydroshikimate Biosynthesis in Escherichia coli

An engineered Escherichia coli cell factory is created to produce a high titer of DHS as well as an efficient system for the conversion DHS into MA, suggesting that the rational cell factory design of DHS and MA biosynthesis could be a feasible way to complement petrochemical-based chemical processes.

Bifunctional optogenetic switch for improving shikimic acid production in E. coli

A cost effective and light-controlled switch reported here provides important insights into environmentally friendly tools for metabolic pathway regulation and should be applicable to the production of other value-add chemicals.

L-tyrosine metabolic pathway in microorganisms and its application in the biosynthesis of plant-derived natural products

The strategies to promote L-tyrosine production in microbial hosts and the common strategies to produce bioactive compounds in engineered Escherichia coli and Saccharomyces cerevisiae are reviewed to better understand and utilize the L-Tyrosine metabolic pathway for microbial overproduction of diverse valuable aromatic compounds in the future.

Production of Caffeic Acid with Co-fermentation of Xylose and Glucose by Multi-modular Engineering in Candida glycerinogenes.

This is the first report to produce CA in C. glycerinogenes with xylose and glucose as carbon sources, which developed a promising strategy for the efficient production of high-value aromatic compounds.

Plant Secondary Metabolites with an Overview of Populus

Compared with bacteria, fewer known pathways produce secondary metabolites in Populus despite P. trichocarpa having had its genome sequenced, leading to more studies of secondary metabolites and their biosynthesis.



Novel technologies combined with traditional metabolic engineering strategies facilitate the construction of shikimate-producing Escherichia coli

This review summarized successful and applicable strategies derived from traditional metabolic engineering and novel technologies for increasing accumulation of shikimate in E. coli.

Innovating a Nonconventional Yeast Platform for Producing Shikimate as the Building Block of High-Value Aromatics.

The development of a new platform based on Scheffersomyces stipitis, whose superior xylose utilization efficiency makes it particularly suited to produce the shikimate group of compounds, which was produced at 3.11 g/L, representing the highest level amongShikimate pathway products in yeasts.

Metabolic engineering for microbial production of shikimic acid.

Metabolic engineering of Escherichia coli for improving shikimate synthesis from glucose.

Shikimic Acid Production in Escherichia coli: From Classical Metabolic Engineering Strategies to Omics Applied to Improve Its Production

The main metabolic engineering strategies that have been applied for the development of efficient SA-producing strains are discussed, as the perspective of omics analysis has focused on further strain improvement for the production of this valuable aromatic intermediate.

Production of shikimic acid from Escherichia coli through chemically inducible chromosomal evolution and cofactor metabolic engineering

It is presented the first demonstration that increasing NADPH availability by overexpressing the pntAB or nadK genes significantly enhances SA production, and an E. coli strain that carries neither a plasmid nor an antibiotic marker was constructed by triclosan-induced chromosomal evolution.

Engineering the shikimate pathway for biosynthesis of molecules with pharmaceutical activities in E. coli.

Tunable switch mediated shikimate biosynthesis in an engineered non-auxotrophic Escherichia coli

The tunable switch developed in this study is an efficient tool for regulating indispensable genes involved in critical metabolic pathways that can conditionally decrease gene expression and substituted it for the original aroK promoters.

Engineering Pseudomonas for phenazine biosynthesis, regulation, and biotechnological applications: a review

The biosynthetic pathway and regulatory mechanism involved in the phenazine biosynthesis are comprehensively discussed, and a summary of biological functionalities and biotechnological applications of the phenazines is provided.

Expanding horizons of shikimic acid

The present review attempts to focus on the market trend of shikimic acid due to its high demand with particular emphasis laid on the pandemics of swine flu.