Metabolic engineering of the baculovirus-expression system via inverse "shotgun" genomic analysis and RNA interference (dsRNA) increases product yield and cell longevity.

@article{Kim2007MetabolicEO,
  title={Metabolic engineering of the baculovirus-expression system via inverse "shotgun" genomic analysis and RNA interference (dsRNA) increases product yield and cell longevity.},
  author={Eun Jeong Kim and Shannon F Kramer and Colin G. Hebert and James R. Valdes and William E. Bentley},
  journal={Biotechnology and bioengineering},
  year={2007},
  volume={98 3},
  pages={645-54}
}
RNA interference (RNAi) is as powerful tool for characterizing gene function in eukaryotic organisms and cultured cell lines. Its use in metabolic engineering has been limited and few reports have targeted protein expression systems to increase yield. In this work, we examine the use of in vitro synthesized double stranded RNA (dsRNA) in the baculovirus… CONTINUE READING