Metabolic engineering of ketocarotenoid formation in higher plants.

@article{Ralley2004MetabolicEO,
  title={Metabolic engineering of ketocarotenoid formation in higher plants.},
  author={Louise Ralley and Eugenia M. A. Enfissi and Norihiko Misawa and Wolfgang W. Schuch and Peter M. Bramley and Paul D Fraser},
  journal={The Plant journal : for cell and molecular biology},
  year={2004},
  volume={39 4},
  pages={477-86}
}
Although higher plants synthesize carotenoids, they do not possess the ability to form ketocarotenoids. In order to generate higher plants capable of synthesizing combinations of ketolated and hydroxylated carotenoids the genes responsible for the carotene 4,4' oxygenase and 3,3' hydroxylase have been transformed into tomato and tobacco. The gene products were produced as a polyprotein. Subsequent cleavage of the polyprotein, targeting of the two enzymes to the plastid and enzyme activities… CONTINUE READING

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