Metabolic engineering of isoflavonoid biosynthesis in alfalfa.

  title={Metabolic engineering of isoflavonoid biosynthesis in alfalfa.},
  author={Bettina E. Deavours and Richard Alastair Dixon},
  journal={Plant physiology},
  volume={138 4},
The potential health benefits of dietary isoflavones have generated considerable interest in engineering the synthesis of these phytoestrogens into plants. Genistein glucoside production (up to 50 nmol g(-1) fresh weight) was engineered in alfalfa (Medicago sativa) leaves by constitutive expression of isoflavone synthase from Medicago truncatula (MtIFS1). Glucosides of biochanin A (4'-O-methylgenistein) and pratensein (3'-hydroxybiochanin A) also accumulated. Although MtIFS1 was highly… CONTINUE READING
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