Metabolic engineering of carotenoid biosynthesis in Escherichia coli by ordered gene assembly in Bacillus subtilis.

@article{Nishizaki2007MetabolicEO,
  title={Metabolic engineering of carotenoid biosynthesis in Escherichia coli by ordered gene assembly in Bacillus subtilis.},
  author={Tomoko Nishizaki and Kenji Tsuge and Mitsuhiro Itaya and Nobuhide Doi and Hiroshi Yanagawa},
  journal={Applied and environmental microbiology},
  year={2007},
  volume={73 4},
  pages={
          1355-61
        }
}
We attempted to optimize the production of zeaxanthin in Escherichia coli by reordering five biosynthetic genes in the natural carotenoid cluster of Pantoea ananatis. Newly designed operons for zeaxanthin production were constructed by the ordered gene assembly in Bacillus subtilis (OGAB) method, which can assemble multiple genes in one step using an intrinsic B. subtilis plasmid transformation system. The highest level of production of zeaxanthin in E. coli (820 microg/g [dry weight]) was… CONTINUE READING

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