Metabolic engineering of arginine permeases to reduce the formation of urea in Saccharomyces cerevisiae.

Abstract

Urea is an important precursor of the harmful carcinogenic product ethyl carbamate in fermented wines. To decipher more fully the contributions of three arginine permeases, Can1p, Gap1p and Alp1p in urea formation, various engineered strains were examined for their ability to form urea. This included seven mutants with different combinations of permease… (More)
DOI: 10.1007/s11274-018-2430-y

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