Metabolic engineering of a phosphoketolase pathway for pentose catabolism in Saccharomyces cerevisiae.

@article{Sonderegger2004MetabolicEO,
  title={Metabolic engineering of a phosphoketolase pathway for pentose catabolism in Saccharomyces cerevisiae.},
  author={Marco Sonderegger and Michael Sch{\"u}mperli and Uwe Sauer},
  journal={Applied and environmental microbiology},
  year={2004},
  volume={70 5},
  pages={2892-7}
}
Low ethanol yields on xylose hamper economically viable ethanol production from hemicellulose-rich plant material with Saccharomyces cerevisiae. A major obstacle is the limited capacity of yeast for anaerobic reoxidation of NADH. Net reoxidation of NADH could potentially be achieved by channeling carbon fluxes through a recombinant phosphoketolase pathway. By heterologous expression of phosphotransacetylase and acetaldehyde dehydrogenase in combination with the native phosphoketolase, we… CONTINUE READING