Metabolic engineering of Lactococcus lactis: influence of the overproduction of alpha-acetolactate synthase in strains deficient in lactate dehydrogenase as a function of culture conditions.

@article{Platteeuw1995MetabolicEO,
  title={Metabolic engineering of Lactococcus lactis: influence of the overproduction of alpha-acetolactate synthase in strains deficient in lactate dehydrogenase as a function of culture conditions.},
  author={C Platteeuw and Jeroen Hugenholtz and Marjo Starrenburg and I J van Alen-Boerrigter and Willem M de Vos},
  journal={Applied and environmental microbiology},
  year={1995},
  volume={61 11},
  pages={3967-71}
}
The als gene for alpha-acetolactate synthase of Lactococcus lactis MG1363 was cloned on a multicopy plasmid under the control of the inducible L. lactis lacA promoter. More than a hundredfold overproduction of alpha-acetolactate synthase was obtained in L. lactis under inducing conditions as compared with that of the host strain, which contained a single chromosomal copy of the als gene. The effect of alpha-acetolactate synthase overproduction on the formation of end products in various L… CONTINUE READING