Metabolic engineering of Escherichia coli for improved 6-deoxyerythronolide B production

@article{Murli2003MetabolicEO,
  title={Metabolic engineering of Escherichia coli for improved 6-deoxyerythronolide B production},
  author={Sumati Murli and Jonathan Kennedy and Linda C. Dayem and John R. Carney and James T. Kealey},
  journal={Journal of Industrial Microbiology and Biotechnology},
  year={2003},
  volume={30},
  pages={500-509}
}
Escherichia coli is an attractive candidate as a host for polyketide production and has been engineered to produce the erythromycin precursor polyketide 6-deoxyerythronolide B (6dEB). In order to identify and optimize parameters that affect polyketide production in engineered E. coli, we first investigated the supply of the extender unit (2S)-methylmalonyl-CoA via three independent pathways. Expression of the Streptomyces coelicolor malonyl/methylmalonyl-CoA ligase (matB) pathway in E. coli… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 35 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 24 references

Metabolic engineering of a methyl - malonyl - CoA mutase - epimerase pathway for complex polyketide biosynthesis in Escherichia coli

  • FC Neidhardt
  • Escherichia coli and Salmonella typhimurium…
  • 2002

Similar Papers

Loading similar papers…