Metabolic engineering of Bacillus subtilis for redistributing the carbon flux to 2,3-butanediol by manipulating NADH levels

@inproceedings{Yang2015MetabolicEO,
  title={Metabolic engineering of Bacillus subtilis for redistributing the carbon flux to 2,3-butanediol by manipulating NADH levels},
  author={Taowei Yang and Zhiming Rao and Guiyuan Hu and Xian Zhang and Mei Liu and Yue Dai and Meijuan Xu and Zhenghong Xu and Shangtian Yang},
  booktitle={Biotechnology for biofuels},
  year={2015}
}
BACKGROUND Acetoin reductase (Acr) catalyzes the conversion of acetoin to 2,3-butanediol (2,3-BD) with concomitant oxidation of NADH to NAD(+). Therefore, intracellular 2,3-BD production is likely governed by the quantities of rate-limiting factor(s) Acr and/or NADH. Previously, we showed that a high level of Acr was beneficial for 2,3-BD accumulation. RESULTS Metabolic engineering strategies were proposed to redistribute carbon flux to 2,3-BD by manipulating NADH levels. The disruption of… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 35 references

On the safety of Bacillus subtilis and B. amyloliquefaciens: a review

Applied Microbiology and Biotechnology • 1991
View 6 Excerpts
Highly Influenced

Microbial 2,3-butanediol production: a state-of-the-art review.

Biotechnology advances • 2011
View 4 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…